CYP8B1 Polyclonal antibody

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  WB analysis using 55217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55217-1-AP (CYP8B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 55217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55217-1-AP (CYP8B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 55217-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55217-1-AP (CYP8B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 55217-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP8B1 antibody (55217-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CYP8B1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 55217-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CP8B, CYP12

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, SMMC-7721 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55217-1-AP targets CYP8B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome P450, family 8, subfamily B, polypeptide 1
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 50-58 kDa
• GenBank Accession Number: NM_004391
• Gene Symbol: CYP8B1
• Gene ID (NCBI): 1582
• RRID: AB_3670243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UNU6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome P450 8B1 (CYP8B1) , also named as CP8B, CYP12 and CYPVIIIB1, is an enzyme required for the synthesis of cholic acid (CA) in the classic bile acid synthesis pathway. CYP8B1 controls biliary balance of cholic acid and chenodeoxycholic acid, ultimately regulating the intestinal absorption of dietary lipids.