CYR61/CCN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CYR61/CCN1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

36

Cat no : 26689-1-AP

Synonyms

CYR61, cysteine-rich protein 61, Cysteine-rich angiogenic inducer 61, Cellular communication network factor 1, CCN1

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Validation Data Gallery

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  WB analysis using 26689-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26689-1-AP (CYR61/CCN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 26689-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26689-1-AP (CYR61/CCN1 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 26689-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26689-1-AP (CYR61/CCN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26689-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26689-1-AP (CYR61/CCN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 26689-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26689-1-AP (CYR61/CCN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 26689-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 26689-1-AP (CYR61/CCN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 26689-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CYR61/CCN1 Polyclonal antibody (26689-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, MDA-MB-453s cells, HeLa cells
• Positive IHC detected in: human colon cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 33 publications below
• IHC: See 8 publications below
• IF: See 8 publications below
• IP: See 1 publications below

Product Information

26689-1-AP targets CYR61/CCN1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYR61/CCN1 fusion protein Ag25129
• Full Name: cysteine-rich, angiogenic inducer, 61
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC001271
• Gene Symbol: CYR61
• Gene ID (NCBI): 3491
• RRID: AB_2880604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYR61 is a member of the CYR61/CTGF/NOV (CCN) protein family. CYR61 participated in cell mitogenesis, cellular adhesion, migration, differentiation, angiogenesis, and survival. Cyr61 is especially associated with diseases related to chronic inflammation, including RA, Crohn's disease and ulcerative colitis. CYR61 may serve essential roles as either an oncogene or a tumor suppressor, depending on the cancer cell type. CYR61 also induces angiogenesis, which supplies oxygen and nutrients to tumors during growth. CYR61 can also play a role in the proliferation, invasion, survival, and metastasis of cancer cells.

Publications

Species	Application	Title
human,mouse	WB,IF	Nat Commun; A translational program that suppresses metabolism to shield the genome.; Authors - Nathan C Balukoff
		ACS Appl Mater Interfaces; A Novel Electrochemiluminescent Immunoassay Based on Target Transformation Assisted with Catalyzed Hairpin Assembly Amplification for the Ultrasensitive Bioassay.; Authors - Hu-Xin Lei
human	WB	Acta Pharmacol Sin; Urolithin A promotes atherosclerotic plaque stability by limiting inflammation and hypercholesteremia in Apolipoprotein E-deficient mice; Authors - Meng-Yun Xu
human	WB	Mol Ther Nucleic Acids; The RNA binding protein QKI5 suppresses ovarian cancer via downregulating transcriptional coactivator TAZ.; Authors - Tao Liu
human	WB	Cell Death Dis; EGFR-PI3K-PDK1 pathway regulates YAP signaling in hepatocellular carcinoma: the mechanism and its implications in targeted therapy.; Authors - Hongwei Xia
human	WB,IHC	Cancers (Basel); WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells.; Authors - Nathalie Ferrand