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CYR61/CCN1 Polyclonal antibody

CYR61/CCN1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26689-1-AP

Synonyms

CCN family member 1, CCN1, CYR61, CYR61/CCN1, cysteine-rich protein 61, GIG1, IBP 10, IGF binding protein 10, IGFBP 10, IGFBP10, Protein CYR61, Protein GIG1



Tested Applications

Positive WB detected inA431 cells, MDA-MB-453s cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

26689-1-AP targets CYR61/CCN1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYR61/CCN1 fusion protein Ag25129
Full Name cysteine-rich, angiogenic inducer, 61
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC001271
Gene symbol CYR61
Gene ID (NCBI) 3491
RRIDAB_2880604
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYR61 is a member of the CYR61/CTGF/NOV (CCN) protein family. CYR61 participated in cell mitogenesis, cellular adhesion, migration, differentiation, angiogenesis, and survival. Cyr61 is especially associated with diseases related to chronic inflammation, including RA, Crohn's disease and ulcerative colitis. CYR61 may serve essential roles as either an oncogene or a tumor suppressor, depending on the cancer cell type. CYR61 also induces angiogenesis, which supplies oxygen and nutrients to tumors during growth. CYR61 can also play a role in the proliferation, invasion, survival, and metastasis of cancer cells.

Protocols

Product Specific Protocols
WB protocol for CYR61/CCN1 antibody 26689-1-APDownload protocol
IHC protocol for CYR61/CCN1 antibody 26689-1-APDownload protocol
IF protocol for CYR61/CCN1 antibody 26689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Nat Commun

A translational program that suppresses metabolism to shield the genome.

Authors - Nathan C Balukoff

ACS Appl Mater Interfaces

A Novel Electrochemiluminescent Immunoassay Based on Target Transformation Assisted with Catalyzed Hairpin Assembly Amplification for the Ultrasensitive Bioassay.

Authors - Hu-Xin Lei
humanWB

Mol Ther Nucleic Acids

The RNA binding protein QKI5 suppresses ovarian cancer via downregulating transcriptional coactivator TAZ.

Authors - Tao Liu
humanWB

Cell Death Dis

EGFR-PI3K-PDK1 pathway regulates YAP signaling in hepatocellular carcinoma: the mechanism and its implications in targeted therapy.

Authors - Hongwei Xia
humanWB,IHC

Cancers (Basel)

WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells.

Authors - Nathalie Ferrand
human,mouseWB

Hum Mol Genet

Abnormal activation of yap/Taz contributes to the pathogenesis of tuberous sclerosis complex.

Authors - Bethany K Terry