CYR61/CCN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CYR61/CCN1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 26689-1-AP

Synonyms

CCN family member 1, CCN1, CYR61, cysteine-rich protein 61, GIG1, IBP 10, IGF binding protein 10, IGFBP 10, IGFBP10, Protein CYR61, Protein GIG1

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Validation Data Gallery

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  WB analysis using 26689-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26689-1-AP (CYR61/CCN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 26689-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26689-1-AP (CYR61/CCN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26689-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26689-1-AP (CYR61/CCN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 26689-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 26689-1-AP (CYR61/CCN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, MDA-MB-453s cells, HeLa cells
• Positive IHC detected in: human colon cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

The immunogen of 26689-1-AP is CYR61/CCN1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYR61/CCN1 fusion protein Ag25129
• Full Name: cysteine-rich, angiogenic inducer, 61
• Calculated molecular weight: 42 kDa
• Observed molecular weight: 42 kDa
• GenBank accession number: BC001271
• Gene symbol: CYR61
• Gene ID (NCBI): 3491
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CYR61 is a member of the CYR61/CTGF/NOV (CCN) protein family. CYR61 participated in cell mitogenesis, cellular adhesion, migration, differentiation, angiogenesis, and survival. Cyr61 is especially associated with diseases related to chronic inflammation, including RA, Crohn's disease and ulcerative colitis. CYR61 may serve essential roles as either an oncogene or a tumor suppressor, depending on the cancer cell type. CYR61 also induces angiogenesis, which supplies oxygen and nutrients to tumors during growth. CYR61 can also play a role in the proliferation, invasion, survival, and metastasis of cancer cells.

Publications

Species	Application	Title
human	WB	Onco Targets Ther; Downregulation of CENPK suppresses hepatocellular carcinoma malignant progression through regulating YAP1.; Authors - Jianlin Wang
human	WB	Cells; Myricetin Suppresses the Propagation of Hepatocellular Carcinoma via Down-Regulating Expression of YAP.; Authors - Minjing Li
human	WB	Cell Discov; Long non-coding RNA MT1DP shunts the cellular defense to cytotoxicity through crosstalk with MT1H and RhoC in cadmium stress.; Authors - Ming Gao ; KD Validated
human	IHC,IF	Biomed Res Int; Expression of YAP/TAZ in Keratocystic Odontogenic Tumors and Its Possible Association with Proliferative Behavior.; Authors - Qi-Wen Man
human	WB,IHC	Exp Mol Pathol; Role of RBM3 in the regulation of cell proliferation in hepatocellular carcinoma.; Authors - Xiaobing Miao
mouse	WB	Environ Toxicol; AMOTL2 restrains transforming growth factor-β1-induced proliferation and extracellular matrix deposition of airway smooth muscle cells via the down-regulation of YAP1 activation.; Authors - Ping Fang