cleaved-Caspase 3 Polyclonal antibody

cleaved-Caspase 3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 25546-1-AP


cleaved-Caspase 3

Tested Applications

Positive WB detected inTunicamycin treated HepG2 cells, Tunicamycin treated HeLa cells, Staurosporine treated Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25546-1-AP targets cleaved-Caspase 3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, sheep, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name caspase 3, apoptosis-related cysteine peptidase
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberNM_032991
Gene Symbol CASP3
Gene ID (NCBI) 836
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 3, also named CPP32, SCA-1, and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7, and -9. CASP3 is involved in the cleavage of huntingtin. The antibody can recognize active-caspase 3.


Product Specific Protocols
WB protocol for cleaved-Caspase 3 antibody 25546-1-APDownload protocol
IHC protocol for cleaved-Caspase 3 antibody 25546-1-APDownload protocol
IF protocol for cleaved-Caspase 3 antibody 25546-1-APDownload protocol
IP protocol for cleaved-Caspase 3 antibody 25546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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