Caspase 6 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Caspase 6 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H7F9

Cat no : 67825-1-PBS

Synonyms

Apoptotic protease Mch 2, CASP 6, CASP6, Caspase 6, Caspase6, MCH2

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Validation Data Gallery

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  WB analysis using 67825-1-Ig (same clone as 67825-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67825-1-Ig (Caspase 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67825-1-Ig (same clone as 67825-1-PBS)

  WB result of Caspase 6 antibody (67825-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 6 transfected Jurkat cells. This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 67825-1-Ig (same clone as 67825-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Caspase 6 antibody (67825-1-Ig, Clone: 1H7F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67825-1-PBS targets Caspase 6 in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caspase 6 fusion protein Ag20534
• Full Name: caspase 6, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 33 kDa, 22 kDa
• Observed Molecular Weight: 33-35 kDa，22kDa, 18kDa
• GenBank Accession Number: BC000305
• Gene Symbol: CASP6
• Gene ID (NCBI): 839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons. The higher molecular mass bands at 49 and 64 kDa likely represent dimers of p28 and p32.(PMID:10438520). In rat testis, it can be detected two bands of 34 kDa and 12 kDa or 14 kDa(PMID:12538628).