Caspase 7 Monoclonal antibody

Caspase 7 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB

Conjugate

Unconjugated

CloneNo.

3C9H4

Cat no : 67956-1-Ig

Synonyms

Apoptotic protease Mch 3, CASP 7, CASP7, Caspase 7, Caspase7, CMH 1, ICE LAP3, ICE like apoptotic protease 3, MCH3

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Validation Data Gallery

• 

  WB analysis using 67956-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67956-1-Ig (Caspase 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67956-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67956-1-Ig (Caspase 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 67956-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67956-1-Ig (Caspase 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, PC-12 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

Sample-dependent, check data in validation data gallery

Product Information

67956-1-Ig targets Caspase 7 in WB applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caspase 7 fusion protein Ag27601
• Full Name: caspase 7, apoptosis-related cysteine peptidase
• Calculated molecular weight: 303 aa, 34 kDa
• Observed molecular weight: 35 kDa
• GenBank accession number: BC015799
• Gene symbol: CASP7
• Gene ID (NCBI): 840
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.