Caspase 7 Monoclonal antibody

Caspase 7 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3C9H4

Cat no : 67956-1-Ig

Synonyms

Apoptotic protease Mch 3, CASP 7, CASP7, Caspase 7, Caspase7, CMH 1, ICE LAP3, ICE like apoptotic protease 3, MCH3



Tested Applications

Positive WB detected inJurkat cells, PC-12 cells, RAW 264.7 cells
Positive IHC detected inrat stomach tissue, human liver cancer tissue, mouse liver tissue, mouse stomach tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67956-1-Ig targets Caspase 7 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Caspase 7 fusion protein Ag27601
Full Name caspase 7, apoptosis-related cysteine peptidase
Calculated Molecular Weight 303 aa, 34 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC015799
Gene Symbol CASP7
Gene ID (NCBI) 840
RRIDAB_2918707
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.

Protocols

Product Specific Protocols
WB protocol for Caspase 7 antibody 67956-1-IgDownload protocol
IHC protocol for Caspase 7 antibody 67956-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols