Caspase 7 Monoclonal antibody, PBS Only

Caspase 7 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C9H4

Cat no : 67956-1-PBS

Synonyms

Apoptotic protease Mch 3, CASP 7, CASP7, Caspase 7, Caspase7, CMH 1, ICE LAP3, ICE like apoptotic protease 3, MCH3

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Validation Data Gallery

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  WB analysis using 67956-1-Ig (same clone as 67956-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67956-1-Ig (Caspase 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 67956-1-Ig (same clone as 67956-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67956-1-Ig (Caspase 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 67956-1-Ig (same clone as 67956-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67956-1-Ig (Caspase 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 67956-1-Ig (same clone as 67956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67956-1-Ig (same clone as 67956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67956-1-Ig (same clone as 67956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 67956-1-Ig (same clone as 67956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 67956-1-Ig (same clone as 67956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 67956-1-Ig (same clone as 67956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 67956-1-Ig (same clone as 67956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67956-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67956-1-PBS targets Caspase 7 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caspase 7 fusion protein Ag27601
• Full Name: caspase 7, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 303 aa, 34 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC015799
• Gene Symbol: CASP7
• Gene ID (NCBI): 840
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.