Collagen Type II Polyclonal antibody

Collagen Type II Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (4)

Applications

WB, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 28459-1-AP

Synonyms

Alpha 1 type II collagen, ANFH, AOM, COL11A3, COL2A1, Collagen alpha 1(II) chain, Collagen Type II, collagen, type II, alpha 1, SEDC



Tested Applications

Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Sample-dependent, check data in validation data gallery

Product Information

28459-1-AP targets Collagen Type II in WB, IHC, IF, CoIP,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human, mouse, rabbit, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Collagen Type II fusion protein Ag29265
Full Name collagen, type II, alpha 1
Calculated molecular weight 134 kDa
GenBank accession numberBC007252
Gene symbol COL2A1
Gene ID (NCBI) 1280
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for Collagen Type II antibody 28459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int J Nanomedicine

Steady Augmentation of Anti-Osteoarthritic Actions of Rapamycin by Liposome-Encapsulation in Collaboration with Low-Intensity Pulsed Ultrasound.

Authors - Chung-Hwan Chen
humanIHC

ACS Biomater Sci Eng

Matrisome Provides a Supportive Microenvironment for Skin Functions of Diverse Species.

Authors - Binghui Liu
HumanWB

Oxid Med Cell Longev

Autophagic Degradation of Gasdermin D Protects against Nucleus Pulposus Cell Pyroptosis and Retards Intervertebral Disc Degeneration In Vivo.

Authors - Zhiwei Liao
ratWB

BMC Musculoskelet Disord

Elevated lymphotoxin-α (TNFβ) is associated with intervertebral disc degeneration.

Authors - Zhu Guo
ratIF, WB

Mol Med Rep

Phgdh serves a protective role in Il‑1β induced chondrocyte inflammation and oxidative‑stress damage.

Authors - Hefei Huang
ratIHC

Pain Res Manag

OPN Deficiency Increases the Severity of Osteoarthritis Associated with Aberrant Chondrocyte Senescence and Apoptosis and Upregulates the Expression of Osteoarthritis-Associated Genes.

Authors - Jian Tian