Collagen Type II Polyclonal antibody

Collagen Type II Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (6)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

117

Reviews (1)

Cat no : 28459-1-AP

Synonyms

Alpha 1 type II collagen, ANFH, AOM, COL11A3, COL2A1, Col2α1, Collagen alpha 1(II) chain, Collagen Type II, collagen, type II, alpha 1, SEDC

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Validation Data Gallery

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  IHC staining of human lung using 28459-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28459-1-AP (Collagen Type II antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 28459-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28459-1-AP (Collagen Type II antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 28459-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using Collagen Type II antibody (28459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human lung cancer using 28459-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using Collagen Type II antibody (28459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human lung cancer tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:800-1:3200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 79 publications below
• IHC: See 46 publications below
• IF: See 40 publications below
• CoIP: See 1 publications below

Product Information

28459-1-AP targets Collagen Type II in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat, pig, rabbit, rabbits
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Collagen Type II fusion protein Ag29265
• Full Name: collagen, type II, alpha 1
• Calculated molecular weight: 134 kDa
• GenBank accession number: BC007252
• Gene symbol: COL2A1
• Gene ID (NCBI): 1280
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	IHC,IF	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
human	WB	Nat Commun; Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis.; Authors - Yun Zhang
human,rat	WB,IF	Mater Today Bio; Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction; Authors - Zhanqiu Dai
human	WB	Mol Ther; Circular RNA circPDE4D Protects against Osteoarthritis by Binding to miR-103a-3p and Regulating FGF18.; Authors - Yizheng Wu
rat	IF	J Control Release; Sialic acid-modified chitosan oligosaccharide-based biphasic calcium phosphate promote synergetic bone formation in rheumatoid arthritis therapy.; Authors - Xiao-Ling Xu
rat	WB,IHC	J Nanobiotechnology; Chitosan oligosaccharides packaged into rat adipose mesenchymal stem cells-derived extracellular vesicles facilitating cartilage injury repair and alleviating osteoarthritis.; Authors - Shenglong Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Balawant (Verified Customer) (12-20-2022)

Ive used this Antibody. this great antibody for IHC.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: colon Tissue