Collagen Type II Polyclonal antibody

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  IHC staining of human lung using 28459-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28459-1-AP (Collagen Type II antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 28459-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28459-1-AP (Collagen Type II antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 28459-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using Collagen Type II antibody (28459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human lung cancer using 28459-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using Collagen Type II antibody (28459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of ATDC-5 using 28459-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed ATDC-5 cells using Collagen Type II antibody (28459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Collagen Type II Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Cat No. 28459-1-AP

Publications(301)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF/ICC, IF-P, CoIP, ELISA

Synonyms

COL2, COL2A1, Alpha 1 type II collagen, Alpha-1 type II collagen, ANFH

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Tested Applications

• Positive IHC detected in: human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human lung cancer tissue
• Positive IF/ICC detected in: ATDC-5 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:800-1:3200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 188 publications below
• IHC: See 118 publications below
• IF: See 124 publications below
• CoIP: See 2 publications below

Product Information

28459-1-AP targets Collagen Type II in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Collagen Type II fusion protein Ag29265
• Full Name: collagen, type II, alpha 1
• Calculated Molecular Weight: 134 kDa
• GenBank Accession Number: BC007252
• Gene Symbol: COL2A1
• Gene ID (NCBI): 1280
• RRID: AB_2881147
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P02458
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The COL2A1 gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found primarily in cartilage and the vitreous humor of the eye. Type II collagen is essential for the formation of the extracellular matrix in cartilage and other connective tissues, providing structure and strength to these tissues .

Publications

Species	Application	Title
mouse,human	IHC,IF	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
rat	IF	Adv Mater; Injectable Microgels with Hybrid Exosomes of Chondrocyte-Targeted FGF18 Gene-Editing and Self-Renewable Lubrication for Osteoarthritis Therapy; Authors - Manyu Chen
rat	IF	Biomaterials; Injectable pathological microenvironment-responsive anti-inflammatory hydrogels for ameliorating intervertebral disc degeneration; Authors - Lei Liu
human	WB	Nat Commun; Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis.; Authors - Yun Zhang
human	WB	J Musculoskelet Neuronal Interact; Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway; Authors - Xiaoming Shi
rabbit	IHC	Acta Biomater; The characterization, cytotoxicity, macrophage response and tissue regeneration of decellularized cartilage.; Authors - Wang Changchen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Balawant (Verified Customer) (12-20-2022)

Ive used this Antibody. this great antibody for IHC.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: colon Tissue