Tested Applications
| Positive IP detected in | TT cells |
| Positive IHC detected in | human lung tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human lung cancer tissue, mouse skin tissue |
| Positive IF/ICC detected in | ATDC-5 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28459-1-AP targets Collagen Type II in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig, rabbit, goat, oryctolagus cuniculus (rabbit) |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29265 Product name: Recombinant human COL2A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-63 aa of BC007252 Sequence: MSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPART Predict reactive species |
| Full Name | collagen, type II, alpha 1 |
| Calculated Molecular Weight | 134 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | BC007252 |
| Gene Symbol | COL2A1 |
| Gene ID (NCBI) | 1280 |
| RRID | AB_2881147 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02458 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The COL2A1 gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found primarily in cartilage and the vitreous humor of the eye. Type II collagen is essential for the formation of the extracellular matrix in cartilage and other connective tissues, providing structure and strength to these tissues. The 200 kDa band is in the form of highly glycosylated procollagen (PMID: 33043724), and the 130-140 kDa band may be mature body protein, excision signal peptide and propeptide, and there are also other fragments or degradation bands of this protein (PMID: 41323462), such as 117 kDa (PMID: 42178419).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Collagen Type II antibody 28459-1-AP | Download protocol |
| IHC protocol for Collagen Type II antibody 28459-1-AP | Download protocol |
| IP protocol for Collagen Type II antibody 28459-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Collagen Type II Polyclonal antibody (Cat# 28459-1-AP) has accumulated 574 citations. Publications span high-impact journals including Nature, Cell Metabolism, Advanced Materials, Nature Communications, Bioactive Materials, Bone Research, Biomaterials, Acta Biomaterialia, Theranostics, and Journal of Orthopaedic Translation, among others. Research fields predominantly cover osteoarthritis, intervertebral disc degeneration, cartilage regeneration, rheumatoid arthritis, ferroptosis, chondrocyte biology, tissue engineering, nanomedicine, and stem cell–based therapies.
| Species | Application | Title |
|---|---|---|
Cell Metab Semaglutide ameliorates osteoarthritis progression through a weight loss-independent metabolic restoration mechanism. | ||
Adv Mater Sequential Targeted Enzyme-Instructed Self-Assembly Supramolecular Nanofibers to Attenuate Intervertebral Disc Degeneration | ||
Adv Mater Mechano-Iontronic Hydrogels Generating Biomimetic Endogenous Bioelectricity for Promoting Cartilage Regeneration. | ||
Adv Mater Spatiotemporally Programming Microenvironment to Recapitulate Endochondral Ossification via Greenhouse-Inspired Bionic Niche | ||
Adv Mater Injectable Microgels with Hybrid Exosomes of Chondrocyte-Targeted FGF18 Gene-Editing and Self-Renewable Lubrication for Osteoarthritis Therapy |
Reviews
What customers say
All three reviews rate the antibody 5 stars. Users report excellent performance across multiple applications, including Western Blot, Immunohistochemistry, Immunofluorescence, and Flow Cytometry. One reviewer used it at 1:700 dilution and described it as working beautifully and essential for research labs. Another successfully detected cells from canine species by flow cytometry while validating it for Western Blot. A third used it at 1:500 dilution on colon tissue and praised it as a great antibody for IHC. Overall, customers are highly satisfied with its reliability and versatility.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mounika (Verified Customer) (01-08-2026) | Antibodies are excellent, worked very beautifully, important for every researh lab!
|
Steve (Verified Customer) (07-23-2025) | I used that antibody in flow to detect cells from canine species, so far so good. I am validating at this moment in Western Blot.
|
Balawant (Verified Customer) (12-20-2022) | Ive used this Antibody. this great antibody for IHC.
|
