Collagen Type II Polyclonal antibody

Collagen Type II Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (4)

Applications

WB, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 28459-1-AP

Synonyms

Alpha 1 type II collagen, ANFH, AOM, COL11A3, COL2A1, Collagen alpha 1(II) chain, Collagen Type II, collagen, type II, alpha 1, SEDC

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Validation Data Gallery

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  IHC staining of human lung using 28459-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28459-1-AP (Collagen Type II antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 28459-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28459-1-AP (Collagen Type II antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:800-1:3200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 14 publications below
• IHC: See 9 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Product Information

28459-1-AP targets Collagen Type II in WB, IHC, IF, CoIP,ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rabbit, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Collagen Type II fusion protein Ag29265
• Full Name: collagen, type II, alpha 1
• Calculated molecular weight: 134 kDa
• GenBank accession number: BC007252
• Gene symbol: COL2A1
• Gene ID (NCBI): 1280
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	IHC	Int J Nanomedicine; Steady Augmentation of Anti-Osteoarthritic Actions of Rapamycin by Liposome-Encapsulation in Collaboration with Low-Intensity Pulsed Ultrasound.; Authors - Chung-Hwan Chen
human	IHC	ACS Biomater Sci Eng; Matrisome Provides a Supportive Microenvironment for Skin Functions of Diverse Species.; Authors - Binghui Liu
Human	WB	Oxid Med Cell Longev; Autophagic Degradation of Gasdermin D Protects against Nucleus Pulposus Cell Pyroptosis and Retards Intervertebral Disc Degeneration In Vivo.; Authors - Zhiwei Liao
rat	WB	BMC Musculoskelet Disord; Elevated lymphotoxin-α (TNFβ) is associated with intervertebral disc degeneration.; Authors - Zhu Guo
rat	IF, WB	Mol Med Rep; Phgdh serves a protective role in Il‑1β induced chondrocyte inflammation and oxidative‑stress damage.; Authors - Hefei Huang
rat	IHC	Pain Res Manag; OPN Deficiency Increases the Severity of Osteoarthritis Associated with Aberrant Chondrocyte Senescence and Apoptosis and Upregulates the Expression of Osteoarthritis-Associated Genes.; Authors - Jian Tian