Common Protease Inhibitor Cocktail (100X)

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  HeLa cells were lysed using RIPA Buffer (PR20135) with and without protease inhibitor cocktail (PR20032). Cell lysates without (lane 2 and 3) and with (lane 1, 4 and 5) the protease inhibitor cocktail were either incubated at 37°C for 4 hours (lanes 2 and 4) or 4°C for 21 hours (lanes 3 and 5). Lysate used for lane 1 did not undergo prolonged incubation. Following this incubation lysates were subjected to SDS-PAGE (5 ug protein/well) followed by western blot with anti-Beta Catenin (51067-2-AP).

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Cat No. PR20032

Publications

53

Concentration

100x

Component

PMSF, E-64, Aprotinin, Bestatin, Leupetin, Pepstatin

Subject

Primary cells, mammalian cultured cells, animal tissues, plant tissues, yeast, or bacteria

Usage

Protein purification, western blotting, immunoprecipitation, immunofluoresence, immunohistochemistry and other studies

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Product Information

Proteintech's Protease Inhibitor Cocktail contains a mixture of protease inhibitors including PMSF, E-64, Aprotinin, Bestatin, Leuptin, and Pepstatin. It mainly inhibits aminopeptidase, cysteine and serine proteases, serine/threonine and tyrosine proteases, aspartate proteases, thermophils, and other metalloendopeptidases. The cocktail can effectively prevent protein degredation during extraction or lysis of primary cells, mammalian cultured cells, animal tissues, plant tissues, yeast, or bacteria. It can be used for multiple applications including protein purification, western blotting, immunoprecipitation, immunofluoresence, immunohistochemistry and other studies.

Components

It contains a mixture of protease inhibitors including PMSF, E-64, Aprotinin, Bestatin, Leuptin, and Pepstatin. 

Storage

Store at -20°C for up to one year.

Usage

Dissolve well at room temperature and add to the lysate or sample solution at a dilution ratio of 1:100 or according to the needs of the experiment.

Notes

1. The protease content can differ across samples. Therefore, the amount of protease inhibitor cocktail can be adjusted according to the experimental situation.

2. This product is a highly concentrated solution. A small amount of precipitation may occur and, therefore, needs to be fully dissolved before use.

3. PMSF is very unstable in aqueous solution, so the cocktail should be added right before use.

4. For your safety and health, please wear a lab coat and disposable gloves.

Cited in Article as

PR20032, Common Protease Inhibitor Cocktail (100X), Proteintech, IL, USA

Publications

Application	Title
	Hum Cell; NR2F1 overexpression alleviates trophoblast cell dysfunction by inhibiting GDF15/MAPK axis in preeclampsia; Authors - Ke Zhang
	FASEB J; Sirt1-Mediated Transcriptional Inhibition of Nr4a3 Alleviates Severe Acute Pancreatitis-Associated Acute Lung Injury; Authors - Xiaolin Dou
	Physiol Behav; Exposure to melamine cyanuric acid in adult mice caused motor activity and skeletal muscle energy metabolism disorder; Authors - Sijie Wang
	Front Pharmacol; Ephedra sinica polysaccharide regulate the anti-inflammatory immunity of intestinal microecology and bacterial metabolites in rheumatoid arthritis; Authors - Yanmiao Ma
	Exp Clin Endocrinol Diabetes; METTL14 Promotes HK-2 Cell Injury in Diabetic Nephropathy Through the Regulation of THBS2 Expression in an m6A-YTHDF2-Dependent Manner; Authors - Qingqing Zhang
	J Biol Chem; IL4I1 overexpression protects against nonalcoholic fatty liver disease in part by inhibiting the AKT/FOXO1 pathway-mediated Th17 cell differentiation; Authors - Yijun Yang