Cullin 5 Polyclonal antibody

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  WB analysis using 31183-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31183-1-AP (Cullin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 31183-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31183-1-AP (Cullin 5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 31183-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31183-1-AP (Cullin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 31183-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31183-1-AP (Cullin 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cullin 5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31183-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CUL5, CUL 5, CUL-5, Cullin-5, VACM 1

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Tested Applications

• Positive WB detected in: LNCaP cells, MCF-7 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31183-1-AP targets Cullin 5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34620

  Product name: Recombinant human CUL5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC063306

  Sequence: MATSNLLKNKGSLQFEDKWDFMRPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLM

• Full Name: cullin 5
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 80 kDa, 90 kDa
• GenBank Accession Number: BC063306
• Gene Symbol: Cullin 5
• Gene ID (NCBI): 8065
• RRID: AB_3669887
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q93034
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cullin family proteins are scaffold proteins for the RING finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. In humans, there are eight members of cullins, including CUL1, 2, 3, 4A, 4B, 5, 7, and CUL9 with an evolutionarily conserved cullin homology domain at the C-terminus, responsible for their interaction with the RING protein, assembling 8 cullin-based RING dependent E3 ubiquitin ligases to target thousands of substrates for ubiquitination and degradation (PMID: 32334051). Cullin 5 is the key scaffolding protein of Cullin RING ligase-5 (CRL-5). Cullin 5 plays positive roles in regulating cell growth, proliferation and physiological and other processes in the human body (PMID: 32194910). Cullin 5 has been detected in the form of double bands in the results of Western blot, which might be caused by neddylation (PMID: 36449095; 31294695).