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Cytochrome c Monoclonal antibody

Cytochrome c Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a


human, mouse, rat and More (1)







Cat no : 66264-1-Ig


CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, human heart tissue, human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, MCF-7 cells, Jurkat cells, HSC-T6 cells, ROS1728 cells, RAW 264.7 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:5000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66264-1-Ig targets Cytochrome c in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cytochrome c fusion protein Ag24349
Full Name cytochrome c, somatic
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 12-15 kDa
GenBank Accession NumberBC009578
Gene Symbol Cytochrome c
Gene ID (NCBI) 54205
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.


Product Specific Protocols
WB protocol for Cytochrome c antibody 66264-1-IgDownload protocol
IHC protocol for Cytochrome c antibody 66264-1-IgDownload protocol
IF protocol for Cytochrome c antibody 66264-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Megha (Verified Customer) (07-10-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Capan-1 cells