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Cytochrome c Monoclonal antibody

Cytochrome c Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2D8D11

Cat no : 66264-1-Ig

Synonyms

CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, human heart tissue, human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, MCF-7 cells, Jurkat cells, HSC-T6 cells, ROS1728 cells, RAW 264.7 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:5000
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66264-1-Ig targets Cytochrome c in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cytochrome c fusion protein Ag24349
Full Name cytochrome c, somatic
Calculated molecular weight 12 kDa
Observed molecular weight 12-15 kDa
GenBank accession numberBC009578
Gene symbol CYCS
Gene ID (NCBI) 54205
RRIDAB_2716798
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.

Protocols

Product Specific Protocols
WB protocol for Cytochrome c antibody 66264-1-IgDownload protocol
IHC protocol for Cytochrome c antibody 66264-1-IgDownload protocol
IF protocol for Cytochrome c antibody 66264-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Hepatology

Hepatic stimulator substance resists hepatic ischemia/reperfusion injury by regulating Drp1 translocation and activation.

Authors - Chao Zhang
humanIF

Nat Commun

Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.

Authors - Wenjun Wang
humanWB

Redox Biol

Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.

Authors - Zhongren Xu
humanIF

J. Pineal Res.

Human transporters, PEPT1/2, facilitate melatonin transportation into mitochondria of cancer cells: an implication of the therapeutic potential.

Authors - Xiaokui Huo
human,mouseWB

Sci China Life Sci

Melatonin decreases GSDME mediated mesothelial cell pyroptosis and prevents peritoneal fibrosis and ultrafiltration failure

Authors - Hongxia Ruan
mouseWB

Br J Pharmacol

Substitution of SERCA2 Cys674 accelerates aortic aneurysm by inducing endoplasmic reticulum stress and promoting cell apoptosis.

Authors - Langtao Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Megha (Verified Customer) (07-10-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Capan-1 cells