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Cytochrome c Monoclonal antibody
Cytochrome c Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
human, mouse, rat and More (1)
WB, IHC, IF, FC, ELISA
Cat no : 66264-1-Ig
Validation Data Gallery
|Positive WB detected in||HeLa cells, HEK-293 cells, human heart tissue, human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, MCF-7 cells, Jurkat cells, HSC-T6 cells, ROS1728 cells, RAW 264.7 cells|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:5000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
66264-1-Ig targets Cytochrome c in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, canine|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Cytochrome c fusion protein Ag24349|
|Full Name||cytochrome c, somatic|
|Calculated molecular weight||12 kDa|
|Observed molecular weight||12-15 kDa|
|GenBank accession number||BC009578|
|Gene ID (NCBI)||54205|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.
|Product Specific Protocols|
|WB protocol for Cytochrome c antibody 66264-1-Ig||Download protocol|
|IHC protocol for Cytochrome c antibody 66264-1-Ig||Download protocol|
|IF protocol for Cytochrome c antibody 66264-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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Megha (Verified Customer) (07-10-2018)