DAAM2 Polyclonal antibody

• Featured Product
• KD/KO Validated

DAAM2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 25206-1-AP

Synonyms

DAAM2, KIAA0381

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Validation Data Gallery

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  WB analysis of mouse brain using 25206-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25206-1-AP (DAAM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 25206-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25206-1-AP (DAAM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 25206-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 25206-1-AP (DAAM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 25206-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25206-1-AP (DAAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 25206-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25206-1-AP (DAAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 25206-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25206-1-AP (DAAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 25206-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25206-1-AP (DAAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 25206-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DAAM2 antibody (25206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, Transfected HEK-293 cells
• Positive IHC detected in: mouse heart tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Product Information

25206-1-AP targets DAAM2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DAAM2 fusion protein Ag18955
• Full Name: dishevelled associated activator of morphogenesis 2
• Calculated Molecular Weight: 1068 aa, 123 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: BC128388
• Gene Symbol: DAAM2
• Gene ID (NCBI): 23500
• RRID: AB_2879959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Front Mol Biosci; Dishevelled-Associated Activator of Morphogenesis 2 (DAAM2) Predicts the Immuno-Hot Phenotype in Pancreatic Adenocarcinoma.; Authors - Qinglin Zhang
human	IHC,WB	Clin Transl Oncol; The prognostic value of DAAM2 in lower grade glioma, liver cancer, and breast cancer; Authors - Zeying Li ; KD Validated
mouse	IHC	Clin Immunol; Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy; Authors - Jie Mei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Erithelgi (Verified Customer) (11-11-2019)

The antibody was verified in an siRNA mediated knockdown

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS osteosarcoma cells