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  • KD/KO Validated

DACH1 Polyclonal antibody

DACH1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 10914-1-AP

Synonyms

DACH, DACH1, Dachshund homolog 1, Dachshund-1



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman oesophagus cancer tissue, human prostate cancer tissue, mouse brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:4000-1:15000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

10914-1-AP targets DACH1 in WB, IP, IHC, IF, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DACH1 fusion protein Ag1354
Full Name dachshund homolog 1 (Drosophila)
Calculated molecular weight 79 kDa
Observed molecular weight 97-100 kDa
GenBank accession numberBC021219
Gene symbol DACH1
Gene ID (NCBI) 1602
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human DACH1.

Protocols

Product Specific Protocols
WB protocol for DACH1 antibody 10914-1-APDownload protocol
IHC protocol for DACH1 antibody 10914-1-APDownload protocol
IF protocol for DACH1 antibody 10914-1-APDownload protocol
IP protocol for DACH1 antibody 10914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC,WB

Stem Cell Reports

Dachshund Depletion Disrupts Mammary Gland Development and Diverts the Composition of the Mammary Gland Progenitor Pool.

Authors - Xuanmao Jiao
  • KO Validated
humanWB,IHC,IF

Oncotarget

Decreased DACH1 expression in glomerulopathy is associated with disease progression and severity.

Authors - Qing-Quan Liu
humanIHC

Front Oncol

CXCL1 as an Unfavorable Prognosis Factor Negatively Regulated by DACH1 in Non-small Cell Lung Cancer.

Authors - Shengnan Yu
humanWB,IHC,IP

EBioMedicine

Organoid modelling identifies that DACH1 functions as a tumour promoter in colorectal cancer by modulating BMP signalling.

Authors - Xiang Hu
  • KO Validated
human,mouseWB,IHC,IF

Oncoimmunology

PMID: 29900050

Authors - Guohui Qin
  • KD Validated
mouseIHC,IF

Am J Physiol Renal Physiol

Global Polysome Analysis of Normal and Injured Podocytes.

Authors - Masahiro Okabe