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DACH1 Polyclonal antibody
DACH1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 10914-1-AP
Validation Data Gallery
|Positive WB detected in||H9C2 cells, HEK-293 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human oesophagus cancer tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:200|
|Sample-dependent, check data in validation data gallery|
10914-1-AP targets DACH1 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebrafish, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DACH1 fusion protein Ag1354|
|Full Name||dachshund homolog 1 (Drosophila)|
|Calculated molecular weight||79 kDa|
|Observed molecular weight||97-110 kDa|
|GenBank accession number||BC021219|
|Gene ID (NCBI)||1602|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human DACH1.
|Product Specific Protocols|
|WB protocol for DACH1 antibody 10914-1-AP||Download protocol|
|IHC protocol for DACH1 antibody 10914-1-AP||Download protocol|
|IF protocol for DACH1 antibody 10914-1-AP||Download protocol|
|IP protocol for DACH1 antibody 10914-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (11-02-2021)
This antibody works great for IF. It specifically marked DACH1-positive coronary vessel cells in mouse heart.