|Positive WB detected in||HEK-293 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human oesophagus cancer tissue, human prostate cancer tissue, mouse brain tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:4000-1:15000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:200|
|Sample-dependent, check data in validation data gallery|
10914-1-AP targets DACH1 in WB, IP, IHC, IF, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DACH1 fusion protein Ag1354|
|Full Name||dachshund homolog 1 (Drosophila)|
|Calculated molecular weight||79 kDa|
|Observed molecular weight||97-100 kDa|
|GenBank accession number||BC021219|
|Gene ID (NCBI)||1602|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human DACH1.
|Product Specific Protocols|
|WB protocol for DACH1 antibody 10914-1-AP||Download protocol|
|IHC protocol for DACH1 antibody 10914-1-AP||Download protocol|
|IF protocol for DACH1 antibody 10914-1-AP||Download protocol|
|IP protocol for DACH1 antibody 10914-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Stem Cell Reports
Dachshund Depletion Disrupts Mammary Gland Development and Diverts the Composition of the Mammary Gland Progenitor Pool.
Decreased DACH1 expression in glomerulopathy is associated with disease progression and severity.
CXCL1 as an Unfavorable Prognosis Factor Negatively Regulated by DACH1 in Non-small Cell Lung Cancer.
Organoid modelling identifies that DACH1 functions as a tumour promoter in colorectal cancer by modulating BMP signalling.
Am J Physiol Renal Physiol
Global Polysome Analysis of Normal and Injured Podocytes.