DACT1 Polyclonal antibody

DACT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27237-1-AP

Synonyms

DACT1, DAPPER, Dapper homolog 1, DAPPER1, DPR1, FRODO, HDPR1, HNG3, THYEX3

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Validation Data Gallery

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  WB analysis using 27237-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27237-1-AP (DACT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27237-1-AP targets DACT1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DACT1 fusion protein Ag26096
• Full Name: dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis)
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: NM_001079520
• Gene Symbol: DACT1
• Gene ID (NCBI): 51339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DACT1 (Dapper/Frodo) is a member of the Dapper family, which includes three genes: DACT1, DACT2 and DACT3. As a tumor suppressor, DACT1 is downregulated in multiple tumor types, such as liver, colon and breast cancer . DACT1 is an important modulator of Wnt signaling, interacting with key components of the various Wnt transduction pathways. For instance, DACT1 binds to disheveled homolog 1 (Dvl1) to induce the degradation of Dvl1 and the subsequent inhibition of the Wnt/β-catenin pathway . (PMID: 34252542, PMID: 29456669)