DDRGK1 Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 86667-2-RR (same clone as 86667-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86667-2-RR (DDRGK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86667-2-PBS in a different storage buffer formulation.

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  WB analysis using 86667-2-RR (same clone as 86667-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86667-2-RR (DDRGK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86667-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86667-2-RR (same clone as 86667-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDRGK1 antibody (86667-2-RR, Clone: 251582G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86667-2-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02579-1

  The mean DDRGK1 concentration was determined to be 109.2 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load, 36.0 ng/mL in PC-3 cell extract based on a 1.3 mg/mL extract load.

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  Cytometric bead array standard curve of MP02579-1

  Cytometric bead array standard curve of MP02579-1, DDRGK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86667-2-PBS. Detection antibody: 86667-1-PBS. Standard: Ag13742. Range: 0.469-60 ng/mL.

DDRGK1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 86667-2-PBS

Clone No.251582G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

C20orf116, Dashurin, DDRGK domain containing 1, DDRGK domain-containing protein 1, UFBP1

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Product Information

86667-2-PBS targets DDRGK1 as part of a matched antibody pair:

MP02579-1: 86667-2-PBS capture and 86667-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13742

  Product name: Recombinant human DDRGK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-314 aa of BC000643

  Sequence: MVAPVWYLVAAALLVGFILFLTRSRGRAASAGQEPLHNEELAGAGRVAQPGPLEPEEPRAGGRPRRRRDLGSRLQAQRRAQRVAWAEADENEEEAVILAQEEEGVEKPAETHLSGKIGAKKLRKLEEKQARKAQREAEEAEREERKRLESQREAEWKKEEERLRLEEEQKEEEERKAREEQAQREHEEYLKLKEAFVVEEEGVGETMTEEQSQSFLTEFINYIKQSKVVLLEDLASQVGLRTQDTINRIQDLLAEGTITGVIDDRGKFIYITPEELAAVANFIRQRGRVSIAELAQASNSLIAWGRESPAQAPA

• Full Name: DDRGK domain containing 1
• Calculated Molecular Weight: 314 aa, 36 kDa
• Observed Molecular Weight: 36-46 kDa
• GenBank Accession Number: BC000643
• Gene Symbol: DDRGK1
• Gene ID (NCBI): 65992
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96HY6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DDRGK1, also name as C20orf116, is a member of the DDRGK domain-containing protein family with unknown function. Depletion of DDRGK1 inhibits cell proliferation and invasion. Expression of NF-κB target genes showed the most significant decrease after depleting of DDRGK1, suggesting that DDRGK1 may play an important role in the NF-κB signaling pathway. DDRGK1 interacts with IκBα and regulates its stability, thereby regulates the NF-κB transcriptional activity. DDRGK1 acts as an important regulator of the NF-κB pathway. (PMID: 23675531)