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DEF6 Antibody

DEF6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11369-1-AP

Synonyms

DEF 6, DEF6, IBP, IRF4 binding protein, SLAT



Tested Applications

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inmouse spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11369-1-AP targets DEF6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DEF6 fusion protein Ag1925
Full Name differentially expressed in FDCP 6 homolog (mouse)
Calculated molecular weight 74 kDa
Observed molecular weight 74 kDa
GenBank accession number BC017504
Gene symbol DEF6
Gene ID (NCBI) 50619
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DEF6 antibody 11369-1-APDownload protocol
IHC protocol for DEF6 antibody 11369-1-APDownload protocol
IP protocol for DEF6 antibody 11369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols