Validation Data Gallery
|Positive WB detected in||HepG2 cells|
|Positive IHC detected in||human small intestine tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
18057-1-AP targets DEFA1 in WB, IHC, IF, CoIP applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||DEFA1 fusion protein Ag12592|
|Full Name||defensin, alpha 1|
|Calculated molecular weight||94 aa, 10 kDa|
|Observed molecular weight||6 kDa|
|GenBank accession number||BC093791|
|Gene ID (NCBI)||1667|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
DEFA1B inhibits ZIKV replication and retards cell cycle progression through interaction with ORC1.
Histone deacetylase 6-mediated downregulation of TMEM100 expedites the development and progression of non-small cell lung cancer.
Syst Biol Reprod Med
Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts.