DEFA1 Polyclonal antibody

DEFA1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18057-1-AP

Synonyms

DEF1, DEFA1, DEFA1B, DEFA2, defensin, alpha 1, HNP 1, HNP 2, HP 1, HP 2, HP1, HP2, MRS, Neutrophil defensin 1



Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman small intestine tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

18057-1-AP targets DEFA1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DEFA1 fusion protein Ag12592
Full Name defensin, alpha 1
Calculated molecular weight 94 aa, 10 kDa
Observed molecular weight 6 kDa
GenBank accession numberBC093791
Gene symbol DEFA1
Gene ID (NCBI) 1667
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DEFA1 antibody 18057-1-APDownload protocol
IHC protocol for DEFA1 antibody 18057-1-APDownload protocol
IF protocol for DEFA1 antibody 18057-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Syst Biol Reprod Med

Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts.

Authors - Genhai Zhu
humanWB,CoIP

Life Sci

DEFA1B inhibits ZIKV replication and retards cell cycle progression through interaction with ORC1.

Authors - Shuang Li