DEPDC6 Polyclonal antibody, PBS Only

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  WB analysis of HeLa using 21312-1-AP (same clone as 21312-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-1080 using 21312-1-AP (same clone as 21312-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 21312-1-AP (same clone as 21312-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 21312-1-AP (same clone as 21312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer slide using 21312-1-AP (DEPDC6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 21312-1-AP (same clone as 21312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer slide using 21312-1-AP (DEPDC6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

DEPDC6 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 21312-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

21312-1-PBS targets DEPDC6 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15959

  Product name: Recombinant human DEPDC6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 60-409 aa of BC024746

  Sequence: YPNCFVAKELIDWLIEHKEASDRETAIKLMQKLADRGIIHHVCDEHKEFKDVKLFYRFRKDDGTFPLDNEVKAFMRGQRLYEKLMSPENTLLQPREEEGVKYERTFMASEFLDWLVQEGEATTRKEAEQLCHRLMEHGIIQHVSSKHPFVDSNLLYQFRMNFRRRRRLMELLNEKSPSSQETHDSPFCLRKQSHDNRKSTSFMSVSPSKEIKIVSAVRRSSMSSCGSSGYFSSSPTLSSSPPVLCNPKSVLKRPVTSEELLTPGAPYARKTFTIVGDAVGWGFVVRGSKPCHIQAVDPSGPAAAAGMKVCQFVVSVNGLNVLHVDYRTVSNLILTGPRTIVMEVMEELEC

• Full Name: DEP domain containing 6
• Calculated Molecular Weight: 409 aa, 46 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC024746
• Gene Symbol: DEPDC6
• Gene ID (NCBI): 64798
• RRID: AB_2878837
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TB45
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DEPTOR, also named as DEPDC6, is a negative regulator of the mTORC1 and mTORC2 signaling pathways. Through mTOR interactions, DEPTOR is implicated in pathways for cell growth and differentiation, as well as cell-cycle prolongation or termination. It inhibits the kinase activity of both complexes. The MW will migrate to 50-55 kDa with phospho- form.