DGAT2 Polyclonal antibody

DGAT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17100-1-AP

Synonyms

DGAT2, Diglyceride acyltransferase 2, HMFN1045, UNQ738/PRO1433

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Validation Data Gallery

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  WB analysis using 17100-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17100-1-AP (DGAT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17100-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17100-1-AP (DGAT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 17100-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17100-1-AP (DGAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse adipose tissue, L02 cells, MCF-7 cells, Neuro-2a cells, mouse heart tissue, mouse liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

17100-1-AP targets DGAT2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DGAT2 fusion protein Ag10719
• Full Name: diacylglycerol O-acyltransferase homolog 2 (mouse)
• Calculated Molecular Weight: 388 aa, 44 kDa
• Observed Molecular Weight: 39 kDa, 44kDa
• GenBank Accession Number: BC015234
• Gene Symbol: DGAT2
• Gene ID (NCBI): 84649
• RRID: AB_2918049
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Diacylglycerol acyltransferase 2 (DGAT2) is an integral membrane protein and major enzyme that catalyzes the final step of triacylglycerol (TG) synthesis in eukaryotes (PMID: 21680734). DGAT2 is present in mitochondria-associated membranes, specialized domains of the ER that are highly enriched in lipid synthetic enzymes (PMID: 19049983). DGAT2 isoforms (39 kDa, 44kDa) have major differences in acyl-CoA substrate specificity toward erucoyl-CoA.

Publications

Species	Application	Title
mouse	WB	Ecotoxicol Environ Saf; Heat stress affects fetal brain and intestinal function associated with the alterations of placental barrier in late pregnant mouse.; Authors - Huiduo Guo
rat	WB	J Appl Microbiol; Weizmannia coagulans JA845 improves atherosclerosis induced by vitamin D3 and high-fat diet in rats through modulating lipid metabolism, oxidative stress, and endothelial vascular injury; Authors - Liying Ma
human	IF	Cell Death Differ; Lipid overload-induced RTN3 activation leads to cardiac dysfunction by promoting lipid droplet biogenesis; Authors - Dong Guo