Filter: AllWBIPIHCIF/ICC

WB analysis using 15194-1-AP (same clone as 15194-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 15194-1-AP (same clone as 15194-1-PBS)
WB result of DHFR antibody (15194-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHFR transfected HeLa cells. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 15194-1-AP (same clone as 15194-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 15194-1-AP (same clone as 15194-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.
WB analysis of Raji using 15194-1-AP (same clone as 15194-1-PBS)
Raji cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 15194-1-AP (same clone as 15194-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.
IP experiment of Raji using 15194-1-AP (same clone as 15194-1-PBS)
IP result of anti-DHFR (IP:15194-1-AP, 3ug; Detection:15194-1-AP 1:500) with Raji cells lysate 2000ug. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 15194-1-AP (same clone as 15194-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 15194-1-AP (same clone as 15194-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 15194-1-AP (same clone as 15194-1-PBS)
Immunofluorescent analysis of HeLa cells using 15194-1-AP (DHFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

DHFR Polyclonal antibody, PBS Only

DHFR Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 15194-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

15194-1-PBS targets DHFR in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7331 Product name: Recombinant human DHFR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-187 aa of BC000192 Sequence: MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND Predict reactive species
Full Name	dihydrofolate reductase
Calculated Molecular Weight	21 kDa
Observed Molecular Weight	21 kDa
GenBank Accession Number	BC000192
Gene Symbol	DHFR
Gene ID (NCBI)	1719
RRID	AB_2091587
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P00374
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).