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  • KD/KO Validated

DHRS2 Polyclonal antibody

DHRS2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC

Conjugate

Unconjugated

Cat no : 15735-1-AP

Synonyms

DHRS2, Dicarbonyl reductase HEP27, HEP27, Protein D, SDR25C1



Tested Applications

Positive WB detected inSKOV-3 cells, human liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

15735-1-AP targets DHRS2 in WB, IP, IHC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHRS2 fusion protein Ag8508
Full Name dehydrogenase/reductase (SDR family) member 2
Calculated molecular weight 300 aa, 32 kDa
Observed molecular weight 27-30 kDa
GenBank accession numberBC007339
Gene symbol DHRS2
Gene ID (NCBI) 10202
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DHRS2(Dehydrogenase/reductase SDR family member 2) is also named as dicarbonyl reductase HEP27, Protein D and belongs to the short-chain dehydrogenases/reductases (SDR) family. It displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates and there is no reductase activity displayed in vitro with steroids, retinoids and sugars as substrates. This protein also may inhibit cell replication. It has 2 isoforms produced by alternative splicing with the molecular weight of 27 kDa and 29 kDa.

Protocols

Product Specific Protocols
WB protocol for DHRS2 antibody 15735-1-APDownload protocol
IHC protocol for DHRS2 antibody 15735-1-APDownload protocol
IP protocol for DHRS2 antibody 15735-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.

Authors - Yunshang Chen
humanWB

Oncol Rep

PMID: 31436301

Authors - Ji-Min Li
  • KD Validated
humanWB,IHC

Epigenetics

Decreased DHRS2 expression is associated with HDACi resistance and poor prognosis in ovarian cancer.

Authors - Yingyan Han
  • KD Validated