DIABLO Polyclonal antibody

• Featured Product
• KD/KO Validated

DIABLO Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (1)

Cat no : 10434-1-AP

Synonyms

DIABLO, diablo homolog (Drosophila), Diablo homolog, mitochondrial, DIABLO S, SMAC, SMAC3

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Validation Data Gallery

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  WB analysis using 10434-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10434-1-AP (DIABLO antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10434-1-AP

  WB result of DIABLO antibody (10434-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIABLO transfected HeLa cells.

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  IP experiment of HepG2 using 10434-1-AP

  IP result of anti-DIABLO(IP:10434-1-AP, 4ug; Detection:10434-1-AP 1:2000) with HepG2 cells lysate 880 ug.

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  IHC staining of human testis using 10434-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10434-1-AP (DIABLO antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10434-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10434-1-AP (DIABLO antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 10434-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10434-1-AP (DIABLO antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10434-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10434-1-AP (DIABLO antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 10434-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10434-1-AP (DIABLO antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10434-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10434-1-AP (DIABLO antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10434-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10434-1-AP (DIABLO antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10434-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10434-1-AP (DIABLO antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10434-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DIABLO antibody (10434-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 10434-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human DIABLO (10434-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, mouse testis tissue, rat testis tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below

Product Information

10434-1-AP targets DIABLO in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DIABLO fusion protein Ag0696
• Full Name: diablo homolog (Drosophila)
• Calculated molecular weight: 27 kDa
• Observed molecular weight: 20 kDa
• GenBank accession number: BC011909
• Gene symbol: DIABLO
• Gene ID (NCBI): 56616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DIABLO, also named as SMAC, promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. DIABLO inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. This antibody recognizes all the three isoforms (27 kDa and 22 kDa) of DIABLO. The highest expression was in the testis, adrenal gland, and ears.

Publications

Species	Application	Title
human	WB	Eur J Med Chem; PKM2/PDK1 dual-targeted shikonin derivatives restore the sensitivity of EGFR-mutated NSCLC cells to gefitinib by remodeling glucose metabolism; Authors - Hongyan Lin
mouse	WB	J Cell Physiol; Resveratrol ameliorates podocyte damage in diabetic mice via SIRT1/PGC-1α mediated attenuation of mitochondrial oxidative stress.; Authors - Tao Zhang
	WB	Ann Transl Med; Botrychium schaffneri Underw. extract acts via DIABLO to induce apoptosis and inhibit proliferation of non-small cell lung carcinoma in vitro and in vivo.; Authors - Yu-Jia Liu
human	WB	Chin Med; Shikonin and 4-hydroxytamoxifen synergistically inhibit the proliferation of breast cancer cells through activating apoptosis signaling pathway in vitro and in vivo.; Authors - Hong-Yan Lin
human	WB	Acta Biochim Biophys Sin (Shanghai); Cathelicidin PR-39 peptide inhibits hypoxia/reperfusion-induced kidney cell apoptosis by suppression of the endoplasmic reticulum-stress pathway.; Authors - Jing Liu
rat	IHC	Mol Med Rep; Mechanisms underlying the protective effect of tannic acid against arsenic trioxide‑induced cardiotoxicity in rats: Potential involvement of mitochondrial apoptosis.; Authors - Yucong Xue

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SHUBHAM (Verified Customer) (03-14-2019)

good

• Applications: Western Blot,
• Cell Tissue Type: AC16

DIABLO Antibody Western Blot, validation ( dilution) in AC16 (Cat no:10434-1-AP)