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  • KD/KO Validated

DIABLO Polyclonal antibody

DIABLO Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10434-1-AP

Synonyms

DIABLO, diablo homolog (Drosophila), Diablo homolog, mitochondrial, DIABLO S, SMAC, SMAC3



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, HepG2 cells, mouse testis tissue, rat testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10434-1-AP targets DIABLO in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DIABLO fusion protein Ag0696
Full Name diablo homolog (Drosophila)
Calculated molecular weight 27 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC011909
Gene symbol DIABLO
Gene ID (NCBI) 56616
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DIABLO, also named as SMAC, promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. DIABLO inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. This antibody recognizes all the three isoforms (27 kDa and 22 kDa) of DIABLO. The highest expression was in the testis, adrenal gland, and ears.

Protocols

Product Specific Protocols
WB protocol for DIABLO antibody 10434-1-APDownload protocol
IHC protocol for DIABLO antibody 10434-1-APDownload protocol
IF protocol for DIABLO antibody 10434-1-APDownload protocol
IP protocol for DIABLO antibody 10434-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Med Sci Monit

Resveratrol Reduces Oxidative Stress and Apoptosis in Podocytes via Sir2-Related Enzymes, Sirtuins1 (SIRT1)/Peroxisome Proliferator-Activated Receptor γ Co-Activator 1α (PGC-1α) Axis.

Authors - Tao Zhang
humanWB

Oncol Lett

Phloretin induces apoptosis of human esophageal cancer via a mitochondria-dependent pathway.

Authors - Hongtao Duan
ratIHC

Mol Med Rep

Mechanisms underlying the protective effect of tannic acid against arsenic trioxide‑induced cardiotoxicity in rats: Potential involvement of mitochondrial apoptosis.

Authors - Yucong Xue
humanWB

Acta Biochim Biophys Sin (Shanghai)

Cathelicidin PR-39 peptide inhibits hypoxia/reperfusion-induced kidney cell apoptosis by suppression of the endoplasmic reticulum-stress pathway.

Authors - Jing Liu
humanWB

Chin Med

Shikonin and 4-hydroxytamoxifen synergistically inhibit the proliferation of breast cancer cells through activating apoptosis signaling pathway in vitro and in vivo.

Authors - Hong-Yan Lin
mouseWB

J Cell Physiol

Resveratrol ameliorates podocyte damage in diabetic mice via SIRT1/PGC-1α mediated attenuation of mitochondrial oxidative stress.

Authors - Tao Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

SHUBHAM (Verified Customer) (03-14-2019)

good

  • Applications: Western Blot,
  • Cell Tissue Type: AC16
DIABLO Antibody Western Blot, validation ( dilution) in AC16 (Cat no:10434-1-AP)