|Positive WB detected in||HEK-293 cells, HeLa cells, Jurkat cells, HepG2 cells, mouse testis tissue, rat testis tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human testis tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10434-1-AP targets DIABLO in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DIABLO fusion protein Ag0696|
|Full Name||diablo homolog (Drosophila)|
|Calculated molecular weight||27 kDa|
|Observed molecular weight||20 kDa|
|GenBank accession number||BC011909|
|Gene ID (NCBI)||56616|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
DIABLO, also named as SMAC, promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. DIABLO inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. This antibody recognizes all the three isoforms (27 kDa and 22 kDa) of DIABLO. The highest expression was in the testis, adrenal gland, and ears.
|Product Specific Protocols|
|WB protocol for DIABLO antibody 10434-1-AP||Download protocol|
|IHC protocol for DIABLO antibody 10434-1-AP||Download protocol|
|IF protocol for DIABLO antibody 10434-1-AP||Download protocol|
|IP protocol for DIABLO antibody 10434-1-AP||Download protocol|
|FC protocol for DIABLO antibody 10434-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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SHUBHAM (Verified Customer) (03-14-2019)