Tested Applications
| Positive WB detected in | HUVEC cells, MCF-7 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10365-1-AP targets DKK3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0417 Product name: Recombinant human DKK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 37-350 aa of BC007660 Sequence: ALSYPQEEATLNEMFREVEELMEDTQHKLRSAVEEMEAEEAAAKASSEVNLANLPPSYHNETNTDTKVGNNTIHVHREIHKITNNQTGQMVFSETVITSVGDEEGRRSHECIIDEDCGPSMYCQFASFQYTCQPCRGQRMLCTRDSECCGDQLCVWGHCTKMATRGSNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPASRLLDLITWELEPDGALDRCPCASGLLCQPHSHSLVYVCKPTFVGSRDQDGEILLPREVPDEYEVGSFMEEVRQELEDLERSLTEEMALGEPAAAAAALLGGEEI Predict reactive species |
| Full Name | dickkopf homolog 3 (Xenopus laevis) |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 38-55 kDa |
| GenBank Accession Number | BC007660 |
| Gene Symbol | DKK3 |
| Gene ID (NCBI) | 27122 |
| RRID | AB_2261615 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBP4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DKK3, also named as REIC, belongs to the dickkopf family. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer's disea. The adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. It has also become evident that some human cancers are resistant to Ad-REIC-induced apoptosis. The DKK3 is a Glycosylation protein with MW about 55 kDa or 38-43 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DKK3 antibody 10365-1-AP | Download protocol |
| WB protocol for DKK3 antibody 10365-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-DKK3 (Cat# 10365-1-AP) has 33 total citations. Key journals include Nature, Nature Cell Biology, Nature Communications, Science Translational Medicine, Journal of Clinical Investigation, Journal of Experimental Medicine, Cancer Letters, Genome Medicine, and British Journal of Cancer. Research fields span oncology (pancreatic, prostate, gastric, renal, colorectal, and oral cancers), Wnt/β-catenin signaling, cardiology, neurology (stroke, Alzheimer's disease), rheumatology, bone/dental biology, ophthalmology, diabetic nephropathy, epigenetics (m6A modification), and veterinary medicine.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function. | ||
Nat Commun A single cell atlas of frozen shoulder capsule identifies features associated with inflammatory fibrosis resolution | ||
Sci Transl Med Suppression of stromal-derived Dickkopf-3 (DKK3) inhibits tumor progression and prolongs survival in pancreatic ductal adenocarcinoma.
| ||
J Clin Invest Phosphatase WIP1 regulates adult neurogenesis and WNT signaling during aging. | ||
Cancer Lett SOX2 promotes tumor metastasis by stimulating epithelial-to-mesenchymal transition via regulation of WNT/β-catenin signal network. |
