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DKK3 Polyclonal antibody

DKK3 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 10365-1-AP

Synonyms

Dickkopf 3, Dickkopf related protein 3, Dkk 3, DKK3, hDkk 3, REIC, RIG



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10365-1-AP targets DKK3 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity canine, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DKK3 fusion protein Ag0417
Full Name dickkopf homolog 3 (Xenopus laevis)
Calculated molecular weight 38 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC007660
Gene symbol DKK3
Gene ID (NCBI) 27122
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DKK3, also named as REIC, belongs to the dickkopf family. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer's disea. The adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. It has also become evident that some human cancers are resistant to Ad-REIC-induced apoptosis. The DKK3 is a Glycosylation protein with MW about 55 kDa or 38-43 kDa.

Protocols

Product Specific Protocols
WB protocol for DKK3 antibody 10365-1-APDownload protocol
IHC protocol for DKK3 antibody 10365-1-APDownload protocol
IF protocol for DKK3 antibody 10365-1-APDownload protocol
FC protocol for DKK3 antibody 10365-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Exp Ther Med

miR-1303 promotes the proliferation, migration and invasion of prostate cancer cells through regulating the Wnt/β-catenin pathway by targeting DKK3.

Authors - Bo Liu
humanWB

Oncol Lett

MicroRNA-183 promotes the proliferation and metastasis of renal cell carcinoma through targeting Dickkopf-related protein 3.

Authors - Xiao-Long Zhang
humanWB,IF

BMC Vet Res

Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines.

Authors - Yuiko Kato
human, canineWB

Vet J

Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours.

Authors - Ochiai Kazuhiko K

Vet Comp Oncol

Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk-3).

Authors - K Ochiai

Sci Rep

Bone secreted factors induce cellular quiescence in prostate cancer cells.

Authors - Li-Yuan Yu-Lee