|Positive WB detected in||mouse brain tissue, human brain tissue, MCF-7 cells|
|Positive IHC detected in||human liver cancer tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10365-1-AP targets DKK3 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||canine, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DKK3 fusion protein Ag0417|
|Full Name||dickkopf homolog 3 (Xenopus laevis)|
|Calculated molecular weight||38 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC007660|
|Gene ID (NCBI)||27122|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
DKK3, also named as REIC, belongs to the dickkopf family. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer's disea. The adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. It has also become evident that some human cancers are resistant to Ad-REIC-induced apoptosis. The DKK3 is a Glycosylation protein with MW about 55 kDa or 38-43 kDa.
|Product Specific Protocols|
|WB protocol for DKK3 antibody 10365-1-AP||Download protocol|
|IHC protocol for DKK3 antibody 10365-1-AP||Download protocol|
|IF protocol for DKK3 antibody 10365-1-AP||Download protocol|
|FC protocol for DKK3 antibody 10365-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Exp Ther Med
miR-1303 promotes the proliferation, migration and invasion of prostate cancer cells through regulating the Wnt/β-catenin pathway by targeting DKK3.
MicroRNA-183 promotes the proliferation and metastasis of renal cell carcinoma through targeting Dickkopf-related protein 3.
BMC Vet Res
Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines.
Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours.
Vet Comp Oncol
Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk-3).
Bone secreted factors induce cellular quiescence in prostate cancer cells.