Tested Applications
| Positive IHC detected in | human stomach cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
27069-1-AP targets DMBT1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16229 Product name: Recombinant human DMBT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1313-1662 aa of BC153299 Sequence: IEVFDGPYRSSPLIARVCDGARGSFTSSSNFMSIRFISDHSITRRGFRAEYYSSPSNDSTNLLCLPNHMQASVSRSYLQSLGFSASDLVISTWNGYYECRPQITPNLVIFTIPYSGCGTFKQADNDTIDYSNFLTAAVSGGIIKRRTDLRIHVSCRMLQNTWVDTMYIANDTIHVANNTIQVEEVQYGNFDVNISFYTSSSFLYPVTSRPYYVDLNQDLYVQAEILHSDAVLTLFVDTCVASPYSNDFTSLTYDLIRSGCVRDDTYGPYSSPSLRIARFRFRAFHFLNRFPSVYLRCKMVVCRAYDPSSRCYRGCVSRSKRDVGSYQEKVDVVLGPIQLQTPPRREEEPR Predict reactive species |
| Full Name | deleted in malignant brain tumors 1 |
| Calculated Molecular Weight | 2413 aa, 261 kDa |
| GenBank Accession Number | BC153299 |
| Gene Symbol | DMBT1 |
| Gene ID (NCBI) | 1755 |
| RRID | AB_2880741 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UGM3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Deleted in malignant brain tumors 1 (DMBT1), also known as the salivary scavenger and agglutinin (SALSA), salivary agglutinin (SAG) or gp340, is a multifunctional molecule with important functions in innate immunity, inflammation and epithelial homeostasis (PMID: 28668353). DMBT1 has some isoforms with the molecular weight of 193, 260 and 340 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DMBT1 antibody 27069-1-AP | Download protocol |
| IHC protocol for DMBT1 antibody 27069-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Discov Breast cancer induces CD62L(+) Kupffer cells via DMBT1 to promote neutrophil extracellular trap formation and liver metastasis. | ||
Front Biosci (Landmark Ed) From Normal Mucosa to Colorectal Cancer in Lynch Syndrome: Single-Cell Dissection of Cellular Heterogeneity and Communication Networks.
|
