DNAJA1 Monoclonal antibody, PBS Only

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  WB analysis using 67184-1-Ig (same clone as 67184-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67184-1-Ig (DNAJA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67184-1-Ig (same clone as 67184-1-PBS)

  WB result of DNAJA1 antibody (67184-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJA1 transfected HeLa cells. This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNAJA1 antibody (67184-1-Ig, Clone: 1B10A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

DNAJA1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67184-1-PBS

Clone No.1B10A10

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HDJ2, hDJ 2, DNAJ2, DjA1, DJ 2

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Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Product Information

67184-1-PBS targets DNAJA1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2284

  Product name: Recombinant human DNAJA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-397 aa of BC008182

  Sequence: MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEKFKQISQAYEVLSDAKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRERRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGVQCQTS

• Full Name: DnaJ (Hsp40) homolog, subfamily A, member 1
• Calculated Molecular Weight: 397 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC008182
• Gene Symbol: DNAJA1
• Gene ID (NCBI): 3301
• RRID: AB_2882480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Euglobulin precipitation
• UNIPROT ID: P31689
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.