TID1 Polyclonal antibody

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  WB analysis of Jurkat using 11088-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11088-1-AP (TID1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 11088-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11088-1-AP (TID1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11088-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11088-1-AP (TID1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 11088-1-AP

  IP result of anti-TID1 (IP:11088-1-AP, 4ug; Detection:11088-1-AP 1:500) with K-562 cells lysate 3200ug.

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  IHC staining of human lung cancer using 11088-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11088-1-AP (TID1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 11088-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11088-1-AP (TID1 antibody) at dilution of 1:100 (under 25x lens).

TID1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 11088-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

DnaJ protein Tid 1, DNAJA3, HCA57, hTid 1, TID 1, TID1

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human lung cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

11088-1-AP targets TID1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TID1 fusion protein Ag1558
• Full Name: DnaJ (Hsp40) homolog, subfamily A, member 3
• Calculated Molecular Weight: 480 aa, 53 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC011855
• Gene Symbol: TID1
• Gene ID (NCBI): 9093
• RRID: AB_2293102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96EY1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	iScience; Role of human HSPE1 for OPA1 processing independent of HSPD1; Authors - Nelson Yeung
human	WB	Nat Commun; Proteogenomic characterization of skull-base chordoma; Authors - Qilin Zhang ; KD Validated