TID1 Polyclonal antibody

TID1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11088-1-AP

Synonyms

DnaJ protein Tid 1, DNAJA3, HCA57, hTid 1, TID 1, TID1



Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

11088-1-AP targets TID1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TID1 fusion protein Ag1558
Full Name DnaJ (Hsp40) homolog, subfamily A, member 3
Calculated molecular weight 480 aa, 53 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC011855
Gene symbol DNAJA3
Gene ID (NCBI) 9093
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TID1 antibody 11088-1-APDownload protocol
IHC protocol for TID1 antibody 11088-1-APDownload protocol
IP protocol for TID1 antibody 11088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Role of human HSPE1 for OPA1 processing independent of HSPD1

Authors - Nelson Yeung