DNAJC5 Polyclonal antibody

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  WB analysis using 32204-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32204-1-AP (DNAJC5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 32204-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 32204-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 32204-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 32204-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 32204-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 32204-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 32204-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNAJC5 antibody (32204-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

DNAJC5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 32204-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ceroid-lipofuscinosis neuronal protein 4, CLN4, CSP, Cysteine string protein, DnaJ (Hsp40) Homolog, Subfamily C, Member 5

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human placenta tissue, mouse cerebellum tissue, mouse lung tissue, mouse pancreas tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32204-1-AP targets DNAJC5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35443

  Product name: Recombinant human DNAJC5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 110-198 aa of NM_025219.2

  Sequence: FVFCGLLTCCYCCCCLCCCFNCCCGKCKPKAPEGEETEFYVSPEDLEAQLQSDEREATDTPIVIQPASATETTQLTADSHPSYHTDGFN

• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 5
• Calculated Molecular Weight: 22kDa，198aa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: NM_025219.2
• Gene Symbol: DNAJC5
• Gene ID (NCBI): 80331
• RRID: AB_3670222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H3Z4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJC5 (DnaJ homolog subfamily C member 5), also known as cysteine string protein α (CSPα), is a member of the HSP40 (DNAJ) family of proteins, as well as a co-chaperone of HSC70, and has been shown to control the extracellular release of many neurodegenerative disease proteins. DNAJC5 contains three structural domains - -a common N-terminal J structural domain conserved in DNAJ proteins, the cysteine string (CS) central structural domain, which is highly palmitoylated and anchors the protein to late endosomes, and an overall disordered C-terminal structural domain. Mutations in DNAJC5 have been strongly implicated in the development of human diseases such as neuronal waxy lipofuscinosis, cystic fibrosis, Huntington's disease and Parkinson's disease.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christine (Verified Customer) (08-05-2025)

Tried at 1:5,000 on total cell lysates of HEK293T transfected with GFP-CSP. Very good detection of the transfected protein (both palmitoylated and unpalmitoylated) and of the endogenous CSP protein. Really good antibody (could be diluted further).

• Applications: Western Blot
• Primary Antibody Dilution: 1:5,000
• Cell Tissue Type: Transfected HEK293T