DNTTIP1 Polyclonal antibody

DNTTIP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11637-1-AP

Synonyms

Terminal deoxynucleotidyltransferase-interacting factor 1, TdT-interacting factor 1, TdIF1, DNTTIP 1, Deoxynucleotidyltransferase terminal-interacting protein 1

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Validation Data Gallery

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  WB analysis of HeLa cells using 11637-1-AP

  HeLa cell were subjected to SDS PAGE followed by western blot with 11637-1-AP (DNTTIP1 Antibody) at dilution of 1:500.

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  IP experiment of HeLa using 11637-1-AP

  IP result of anti-DNTTIP1 (IP:11637-1-AP, 4ug; Detection:11637-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IP experiment of HeLa using 11637-1-AP

  IP result of anti-DNTTIP1 (IP:11637-1-AP, 4ug; Detection:11637-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 11637-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNTTIP1 antibody (11637-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11637-1-AP targets DNTTIP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNTTIP1 fusion protein Ag2213
• Full Name: deoxynucleotidyltransferase, terminal, interacting protein 1
• Calculated Molecular Weight: 329 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC024290
• Gene Symbol: DNTTIP1
• Gene ID (NCBI): 116092
• RRID: AB_2877787
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNTTIP1, also named as Deoxynucleotidyltransferase terminal-interacting protein 1, is a 329 amino acid protein ,which contains a Nuclear localization signal and localizes in the nuclear. DNTTIP1 also acts as a transcriptional regulator, which binding to the consensus sequence 5'-GNTGCATG-3' following an AT-tract. Associates with RAB20 promoter and positively regulates its transcription.