DPY30 Polyclonal antibody

DPY30 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16281-1-AP

Synonyms

dpy 30 homolog (C. elegans), Dpy 30 like protein, Dpy 30L, DPY30, HDPY 30, Protein dpy 30 homolog, Saf19

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Validation Data Gallery

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  IHC staining of human stomach cancer using 16281-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16281-1-AP (DPY30 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 16281-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16281-1-AP (DPY30 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 16281-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16281-1-AP (DPY30 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 16281-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DPY30 antibody (16281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive IHC detected in: human stomach cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16281-1-AP targets DPY30 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DPY30 fusion protein Ag9370
• Full Name: dpy-30 homolog (C. elegans)
• Calculated Molecular Weight: 99 aa, 11 kDa
• GenBank Accession Number: BC015970
• Gene Symbol: DPY30
• Gene ID (NCBI): 84661
• RRID: AB_2918044
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	PLoS Biol; A novel TOX3-WDR5-ABCG2 signaling axis regulates the progression of colorectal cancer by accelerating stem-like traits and chemoresistance; Authors - Jiaojiao Hao