DRAM Polyclonal antibody

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  WB analysis using 31777-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31777-1-AP (DRAM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

DRAM Polyclonal Antibody for WB, ELISA

Cat No. 31777-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DRAM1, DNA damage-regulated autophagy modulator protein 1, Damage-regulated autophagy modulator

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Tested Applications

• Positive WB detected in: HCT 116 cells, HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31777-1-AP targets DRAM in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DRAM fusion protein Ag33711
• Full Name: damage-regulated autophagy modulator
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC018435
• Gene Symbol: DRAM
• Gene ID (NCBI): 55332
• RRID: AB_3670110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8N682
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Damage-regulated autophagy modulator (DRAM) is a lysosomal membrane protein of autophagy that enriches in lung tissue and plays a central role in p53/TP53-mediated apoptosis (PMID: 16839881), which increased autophagy flux by promoting lysosomal acidification and protease activation (PMID: 23696801). It has 238 amino acids and six hydrophobic transmembrane regions. Reports show DRAM to be downregulated in squamous cancers, indicating a selective pressure to lose DRAM during tumor development (PMID: 17102582). Overexpression of DRAM1 can promote mitophagy and enhance mitochondrial function (PMID: 31204316).