Desmocollin 2 Monoclonal antibody

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  WB analysis of HEK-293 using 60239-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60239-1-Ig (Desmocollin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 60239-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60239-1-Ig (Desmocollin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60239-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60239-1-Ig (Desmocollin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse heart using 60239-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Desmocollin 2 antibody (60239-1-Ig, Clone: 1H8G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat heart using 60239-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using Desmocollin 2 antibody (60239-1-Ig, Clone: 1H8G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Desmocollin 2 Monoclonal Antibody for WB, IF-P, ELISA

Cat No. 60239-1-Ig

Clone No.1H8G1

Publications(5)

Reviews (2)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

DSC2, 1H8G1, ARVD11, Cadherin family member 2, CDHF2

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Tested Applications

• Positive WB detected in: HEK-293 cells, Raji cells
• Positive IF-P detected in: mouse heart tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

60239-1-Ig targets Desmocollin 2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Desmocollin 2 fusion protein Ag5450
• Full Name: desmocollin 2
• Calculated Molecular Weight: 100 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC063291
• Gene Symbol: Desmocollin 2
• Gene ID (NCBI): 1824
• RRID: AB_2881362
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q02487
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Desmocollin 2 (DSC2) is a member of the desmocollin subfamily of the cadherin superfamily. The desmocollins (DSC1-3), along with the desmogleins (DSG1-4), are found primarily in epithelia and cardiac muscle where they constitute the adhesive proteins of the intercellular desmosome junctions and are required for cell adhesion and desmosome formation. Mutations in DSC2 gene are associated with arrhythmogenic right ventricular dysplasia-11.

Publications

Species	Application	Title
human	WB	J Cell Biol; DPM1 modulates desmosomal adhesion and epidermal differentiation through SERPINB5; Authors - Maitreyi Rathod
human	WB	Acta Physiol (Oxf); Clustering of desmosomal cadherins by desmoplakin is essential for cell-cell adhesion.; Authors - Marie-Therès Wanuske
human	WB	PLoS Pathog; Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of junctional adhesion molecule 1.; Authors - Hiroki Takeuchi
human	IF	Stem Cell Res Ther; Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.; Authors - Ling Leng
human	WB	Mol Ther Oncolytics; Exosomal miR-205-5p enhances angiogenesis and nasopharyngeal carcinoma metastasis by targeting desmocollin-2.; Authors - Wenjuan Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jane (Verified Customer) (02-03-2022)

A very weak and smear band found around the right molecular weight. However, the quality is not good enough to do any analysis or generate results.

• Applications: Western Blot
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Cardiomyoyctes

FH

Jing (Verified Customer) (02-04-2020)

Tested in hiPSC-derived cardiomyocytes, it showed band around 130Kd.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSC-derived cardiomyocytes