Desmocollin 2 Monoclonal antibody
Desmocollin 2 Monoclonal Antibody for FC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse
Applications
WB, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1H8G1
Cat no : 60239-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, Raji cells |
| Positive FC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Flow Cytometry (FC) | FC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
60239-1-Ig targets Desmocollin 2 in WB, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Desmocollin 2 fusion protein Ag5450 |
| Full Name | desmocollin 2 |
| Calculated Molecular Weight | 100 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC063291 |
| Gene Symbol | DSC2 |
| Gene ID (NCBI) | 1824 |
| RRID | AB_2881362 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Desmocollin 2 (DSC2) is a member of the desmocollin subfamily of the cadherin superfamily. The desmocollins (DSC1-3), along with the desmogleins (DSG1-4), are found primarily in epithelia and cardiac muscle where they constitute the adhesive proteins of the intercellular desmosome junctions and are required for cell adhesion and desmosome formation. Mutations in DSC2 gene are associated with arrhythmogenic right ventricular dysplasia-11.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Desmocollin 2 antibody 60239-1-Ig | Download protocol |
| FC protocol for Desmocollin 2 antibody 60239-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Physiol (Oxf) Clustering of desmosomal cadherins by desmoplakin is essential for cell-cell adhesion. | ||
PLoS Pathog Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of junctional adhesion molecule 1. | ||
Stem Cell Res Ther Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth. | ||
Mol Ther Oncolytics Exosomal miR-205-5p enhances angiogenesis and nasopharyngeal carcinoma metastasis by targeting desmocollin-2. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Jane (Verified Customer) (02-03-2022) | A very weak and smear band found around the right molecular weight. However, the quality is not good enough to do any analysis or generate results.
|
FH Jing (Verified Customer) (02-04-2020) | Tested in hiPSC-derived cardiomyocytes, it showed band around 130Kd.
|
