Desmocollin 2 Monoclonal antibody

Desmocollin 2 Monoclonal Antibody for FC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1H8G1

Reviews (1)

Cat no : 60239-1-Ig

Synonyms

ARVD11, Cadherin family member 2, CDHF2, desmocollin 2, Desmocollin 3, Desmosomal glycoprotein II, Desmosomal glycoprotein III, DG2, DGII/III, DSC2, DSC3

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 60239-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60239-1-Ig (Desmocollin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 60239-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60239-1-Ig (Desmocollin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60239-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60239-1-Ig (Desmocollin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HEK-293 using 60239-1-Ig

  1X10^6 HEK-293 cells were stained with 0.20ug Desmocollin 2 antibody (60239-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: HEK-293 cells, Raji cells
• Positive FC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

The immunogen of 60239-1-Ig is Desmocollin 2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Desmocollin 2 fusion protein Ag5450
• Full Name: desmocollin 2
• Calculated molecular weight: 100 kDa
• Observed molecular weight: 110 kDa
• GenBank accession number: BC063291
• Gene symbol: DSC2
• Gene ID (NCBI): 1824
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Desmocollin 2 (DSC2) is a member of the desmocollin subfamily of the cadherin superfamily. The desmocollins (DSC1-3), along with the desmogleins (DSG1-4), are found primarily in epithelia and cardiac muscle where they constitute the adhesive proteins of the intercellular desmosome junctions and are required for cell adhesion and desmosome formation. Mutations in DSC2 gene are associated with arrhythmogenic right ventricular dysplasia-11.

Publications

Species	Application	Title
human	WB	PLoS Pathog; Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of junctional adhesion molecule 1.; Authors - Hiroki Takeuchi
human	IF	Stem Cell Res Ther; Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.; Authors - Ling Leng
human	WB	Mol Ther Oncolytics; Exosomal miR-205-5p enhances angiogenesis and nasopharyngeal carcinoma metastasis by targeting desmocollin-2.; Authors - Wenjuan Yang
human	WB,IP	Acta Physiol (Oxf); Clustering of desmosomal cadherins by desmoplakin is essential for cell-cell adhesion.; Authors - Marie-Therès Wanuske

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jing (Verified Customer) (02-04-2020)

Tested in hiPSC-derived cardiomyocytes, it showed band around 130Kd.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSC-derived cardiomyocytes

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