DUSP10 Polyclonal antibody

DUSP10 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30493-1-AP

Synonyms

DUSP10, MAP kinase phosphatase 5, MKP 5, MKP5

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Validation Data Gallery

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  WB analysis using 30493-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30493-1-AP (DUSP10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30493-1-AP targets DUSP10 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DUSP10 fusion protein Ag32718
• Full Name: dual specificity phosphatase 10
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC031405
• Gene Symbol: DUSP10
• Gene ID (NCBI): 11221
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DUSP10, also known as MKP5, is a member of the MKPs subfamily involved in cell proliferation, differentiation, and migration. DUSP10 is a dual specificity phosphatase capable of dephosphorylating both pTyr and pThr residues of activated MAPKs with different affinities. It has been shown that isoforms of the p38 and JNK subfamilies are selectively and more effectively dephosphorylated than ERK subfamily members. DUSP10 has been proposed as a target for therapeutic treatment of atherosclerosis, since DUSP10 inhibition reduces NF-κB-induced TNF-α expression and increases TGF-β1 levels in this disease (PMID: 30939861).