WB analysis of Jurkat using 12219-1-AP (same clone as 12219-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
Jurkat cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
WB analysis of A375 using 12219-1-AP (same clone as 12219-1-PBS)
A375 cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
A375 cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 12219-1-AP (same clone as 12219-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
human brain tissue were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 12219-1-AP (same clone as 12219-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IP experiment of mouse testis using 12219-1-AP (same clone as 12219-1-PBS)
IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 12219-1-AP (same clone as 12219-1-PBS)
IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with HeLa cells lysate 2080 ug. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with HeLa cells lysate 2080 ug. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IHC staining of mouse stomach using 12219-1-AP (same clone as 12219-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IHC staining of mouse stomach using 12219-1-AP (same clone as 12219-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 12219-1-AP (same clone as 12219-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 12219-1-AP (same clone as 12219-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IHC staining of rat cerebellum using 12219-1-AP (same clone as 12219-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IHC staining of rat cerebellum using 12219-1-AP (same clone as 12219-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IF Staining of A375 using 12219-1-AP (same clone as 12219-1-PBS)
Immunofluorescent analysis of A375 cells using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of A375 cells using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
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WB analysis of Jurkat using 12219-1-AP (same clone as 12219-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
WB analysis of A375 using 12219-1-AP (same clone as 12219-1-PBS)
A375 cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 12219-1-AP (same clone as 12219-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 12219-1-AP (same clone as 12219-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IHC Figures
IHC staining of mouse stomach using 12219-1-AP (same clone as 12219-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IHC staining of mouse stomach using 12219-1-AP (same clone as 12219-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 12219-1-AP (same clone as 12219-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 12219-1-AP (same clone as 12219-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IHC staining of rat cerebellum using 12219-1-AP (same clone as 12219-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IHC staining of rat cerebellum using 12219-1-AP (same clone as 12219-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IP Figures
IP experiment of mouse testis using 12219-1-AP (same clone as 12219-1-PBS)
IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 12219-1-AP (same clone as 12219-1-PBS)
IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with HeLa cells lysate 2080 ug. This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of A375 using 12219-1-AP (same clone as 12219-1-PBS)
Immunofluorescent analysis of A375 cells using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12219-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
DYNC1I2 Polyclonal antibody
Catalog Number
12219-1-PBS
Citations
-
Dilutions
Applications
WB, IHC, IF/ICC, IP, Indirect ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet