DZIP3 Polyclonal antibody

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  WB analysis using 33563-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33563-1-AP (DZIP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 33563-1-AP

  IP result of anti-DZIP3 (IP:33563-1-AP, 4ug; Detection:33563-1-AP 1:1000) with HEK-293T cells lysate 2080 ug.

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  IHC staining of human kidney using 33563-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 33563-1-AP (DZIP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 33563-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 33563-1-AP (DZIP3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 33563-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DZIP3 antibody (33563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 33563-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DZIP3 antibody (33563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DZIP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 33563-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

DAZ-interacting protein 3, E3 ubiquitin-protein ligase DZIP3, EC:2.3.2.27, hRUL138, KIAA0675

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Tested Applications

• Positive WB detected in: AC16 cells
• Positive IP detected in: HEK-293T cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33563-1-AP targets DZIP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35815

  Product name: Recombinant human DZIP3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 631-862 aa of BC063882

  Sequence: AEINKDGTSIPSESSTESLKDLQEVKSKQRKKKKTKNKKNKDSKEDQVPYVVEKEEQLRKEQANPHSVSRLIKDDASDVQEDSAMEDKFYSLDELHILDMIEQGSAGKVTTDYGETEKERLARQRQLYKLHYQCEDFKRQLRTVTFRWQENQMQIKKKDKIIASLNQQVAFGINKVSKLQRQIHAKDNEIKNLKEQLSMKRSQWEMEKHNLESTMKTYVSKLNAETSRALTA

• Full Name: DAZ interacting protein 3, zinc finger
• Calculated Molecular Weight: 1208 aa, 139 kDa
• Observed Molecular Weight: 139-150 kDa
• GenBank Accession Number: BC063882
• Gene Symbol: DZIP3
• Gene ID (NCBI): 9666
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q86Y13
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DZIP3/hRUL138 is a poorly characterized RNA-binding RING E3-ubiquitin ligase with functions in embryonic development. DZIP3 localizes in both the nucleus and cytoplasm (PMID: 33067265). DZIP3 was mainly reported to be an RNA-binding ligase that causes histone H2A monoubiquitination, which represses transcription. DZIP3 also regulates the differentiation of stem cells (PMID: 32796072).