Digoxin Recombinant monoclonal antibody, PBS Only

• 

  ELISA experiment of Digoxin using 86100-7-RR (same clone as 86100-7-PBS)

  ELISA analysis of Digoxin was performed by coating wells of a 96-well plate with 100 µl per well of Digoxin-BSA, diluted in carbonate/bicarbonate buffer, at a concentration of 0.7 µg/mL (2hr,37℃). The plate was washed, blocked with 1% BSA blocking buffer, and incubated with 100 µl per well of Digoxin Recombinant Monoclonal Antibody (Product # 86100-7-RR) and serially diluting it with a starting concentration of 1 µg/mL (1 hr,37℃). The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:10,000 (1 hr, 37℃). Detection was performed using an Ultra TMB Substrate (3-5 min, room temp). The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. This data was developed using the same antibody clone with 86100-7-PBS in a different storage buffer formulation.

Digoxin Uni-rAb^® Recombinant Antibody for ELISA, Indirect ELISA

Cat No. 86100-7-PBS

Clone No.250496G6

Host / Isotype

Rabbit / IgG

Reactivity

-

Applications

ELISA, Indirect ELISA

Synonyms

20830-75-5, CasNo 20830-75-5, 地高辛

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Product Information

86100-7-PBS targets Digoxin in ELISA, Indirect ELISA applications and shows reactivity with - samples.

• Tested Reactivity: -
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: Digoxin
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

This antibody is designed for Digoxin. Digoxin is a cardiac glycoside extracted from the leaves of digitalis (Digitalis lanata). Its mechanism of action mainly involves enhancing myocardial contractility, slowing heart rate, and reducing sympathetic nerve activity.