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E-cadherin Monoclonal antibody
E-cadherin Monoclonal Antibody for FC, IF, IHC, WB,ELISA
Cat no : 60335-1-Ig
Validation Data Gallery
|Positive WB detected in||PC-3 cells, A431 cells, MCF-7 cells, pig brain tissue, MKN-45 cells, SGC-7901 cells|
|Positive IHC detected in||human breast cancer tissue, human colon tissue, rat colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human breast cancer tissue, human kidney tissue|
|Positive FC detected in||A431 cells|
|Western Blot (WB)||WB : 1:2000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
60335-1-Ig targets E-cadherin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, pig, rat samples.
|Tested Reactivity||human, pig, rat|
|Cited Reactivity||human, monkey, mouse, pig|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||E-cadherin fusion protein Ag15085|
|Full Name||cadherin 1, type 1, E-cadherin (epithelial)|
|Calculated molecular weight||882 aa, 97 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC141838|
|Gene ID (NCBI)||999|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.
|Product Specific Protocols|
|WB protocol for E-cadherin antibody 60335-1-Ig||Download protocol|
|IHC protocol for E-cadherin antibody 60335-1-Ig||Download protocol|
|IF protocol for E-cadherin antibody 60335-1-Ig||Download protocol|
|FC protocol for E-cadherin antibody 60335-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joshua (Verified Customer) (12-27-2019)
Caco-2 cells fixed in 4% paraformaldehyde. Stained overnight at 4C. Bright stain, minimal background
Louisiane (Verified Customer) (02-06-2019)
Cells were fixed with 4% PFA for 10 min, permeabilized with 0.1% Triton-X100 for 5 min and blocked with 1% FBS/1% BSA in PBS for 3 h. Antibodies were diluted in 1% FBS/1% BSA in PBS. Primary antibody: 2 h. Alexa Fluor anti-mouse secondary antibody (1:250): 1 h.Cells were imaged by confocal microscopy - no labeling was observed.