CoraLite® Plus 488-conjugated E-cadherin Monoclonal antibody

E-cadherin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6B11F11

Cat no : CL488-60335

Synonyms

Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, Epithelial cadherin, LCAM, UVO, Uvomorulin

---

Validation Data Gallery

• 

  IF Staining of human breast cancer using CL488-60335

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 E-cadherin antibody (CL488-60335, Clone: 6B11F11 ) at dilution of 1:200.

• 

  IF Staining of human breast cancer using CL488-60335

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 E-cadherin antibody (CL488-60335, Clone: 6B11F11 ) at dilution of 1:200.

---

Tested Applications

• Positive IF detected in: human breast cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60335 targets E-cadherin in IF applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: E-cadherin fusion protein Ag15085
• Full Name: cadherin 1, type 1, E-cadherin (epithelial)
• Calculated Molecular Weight: 882 aa, 97 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC141838
• Gene Symbol: CDH1
• Gene ID (NCBI): 999
• RRID: AB_2919249
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.