CoraLite®488-conjugated E-cadherin Monoclonal antibody

E-cadherin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

6B11F11

Cat no : CL488-60335

Synonyms

Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, Epithelial cadherin, LCAM, UVO, Uvomorulin



Tested Applications

Positive IF detected inhuman breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60335-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-60335 targets E-cadherin in IF applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen E-cadherin fusion protein Ag15085
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Calculated molecular weight 882 aa, 97 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC141838
Gene symbol CDH1
Gene ID (NCBI) 999
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

Protocols

Product Specific Protocols
IF protocol for CL488 E-cadherin antibody CL488-60335Download protocol
Standard Protocols
Click here to view our Standard Protocols