E-cadherin Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

E-cadherin Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, rat

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6B11F11

Cat no : 60335-1-PBS

Synonyms

Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVO, Uvomorulin

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Validation Data Gallery

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  WB analysis of A431 using 60335-1-Ig (same clone as 60335-1-PBS)

  WB result of E-cadherin antibody (60335-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E-cadherin transfected A431 cells. This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 60335-1-Ig (same clone as 60335-1-PBS)

  PC-3, MKN-45, SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 60335-1-Ig (same clone as 60335-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 60335-1-Ig (same clone as 60335-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60335-1-Ig (same clone as 60335-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

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  IHC staining of rat stomach using 60335-1-Ig (same clone as 60335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

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  IHC staining of rat stomach using 60335-1-Ig (same clone as 60335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 60335-1-Ig (same clone as 60335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60335-1-Ig (same clone as 60335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60335-1-Ig (same clone as 60335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 60335-1-Ig (same clone as 60335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

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  IF Staining of human kidney using 60335-1-Ig (same clone as 60335-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:300 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (18150-1-AP, green). DNA was stained by DAPI (blue). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 60335-1-Ig (same clone as 60335-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 60335-1-Ig (same clone as 60335-1-PBS)

  1X10^6 A431 cells were stained with 0.2 ug Anti-Human E-cadherin (60335-1-Ig, Clone:6B11F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60335-1-PBS targets E-cadherin in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

• Tested Reactivity: human, pig, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: E-cadherin fusion protein Ag15085
• Full Name: cadherin 1, type 1, E-cadherin (epithelial)
• Calculated Molecular Weight: 882 aa, 97 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC141838
• Gene Symbol: CDH1
• Gene ID (NCBI): 999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.