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E2F1 Monoclonal antibody

E2F1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b


Human, mouse, rat and More (1)







Cat no : 66515-1-Ig


E2F 1, E2F transcription factor 1, E2F1, PBR3, PRB binding protein E2F 1, RBAP 1, RBAP1, RBBP 3, RBBP3, RBP3, Transcription factor E2F1

Tested Applications

Positive WB detected inLNCaP cells, HepG2 cells, HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

66515-1-Ig targets E2F1 in WB, IP, IF, ChIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen E2F1 fusion protein Ag17363
Full Name E2F transcription factor 1
Calculated molecular weight 437 aa, 47 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC050369
Gene symbol E2F1
Gene ID (NCBI) 1869
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.


Product Specific Protocols
WB protocol for E2F1 antibody 66515-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Death Dis

E2F1 mediated DDX11 transcriptional activation promotes hepatocellular carcinoma progression through PI3K/AKT/mTOR pathway.

Authors - Yan Yu

FEBS Open Bio

Augmentation of the therapeutic efficacy of WEE1 kinase inhibitor AZD1775 by inhibiting the YAP-E2F1-DNA damage response pathway axis.

Authors - Yusuke Oku

Cell Biol Int

FOXO1 inhibits prostate cancer cell proliferation via suppressing E2F1 activated NPRL2 expression

Authors - Yu Tang

J Gene Med

Downregulation of lncRNA HCG11 promotes cell proliferation of oral squamous cell carcinoma through sponging miR-455-5p.

Authors - Jingjing Wu

J Gene Med

Long non-coding RNA SNHG1 activates glycolysis to promote hepatocellular cancer progression through the miR-326/PKM2 axis.

Authors - Yue Wang

Front Physiol

Myricanol Inhibits Platelet Derived Growth Factor-BB-Induced Vascular Smooth Muscle Cells Proliferation and Migration in vitro and Intimal Hyperplasia in vivo by Targeting the Platelet-Derived Growth Factor Receptor-β and NF-κB Signaling.

Authors - Siyuan Fan