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E2F1 Monoclonal antibody

E2F1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

CloneNo.

5D7G8

Cat no : 66515-1-Ig

Synonyms

E2F 1, E2F transcription factor 1, E2F1, PBR3, PRB binding protein E2F 1, RBAP 1, RBAP1, RBBP 3, RBBP3, RBP3, Transcription factor E2F1



Tested Applications

Positive WB detected inLNCaP cells, HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66515-1-Ig targets E2F1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen E2F1 fusion protein Ag17363
Full Name E2F transcription factor 1
Calculated molecular weight 437 aa, 47 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC050369
Gene symbol E2F1
Gene ID (NCBI) 1869
RRIDAB_2881878
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.

Protocols

Product Specific Protocols
WB protocol for E2F1 antibody 66515-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cancer Res

LncRNA AGPG confers endocrine resistance in breast cancer by promoting E2F1 activity

Authors - Shiyi Yu
humanWB

Cell Rep

FAK-mediated phosphorylation at Y464 regulates p85β nuclear translocation to promote tumorigenesis of ccRCC by repressing RB1 expression

Authors - Yanhua Zhang
humanWB

Acta Pharmacol Sin

Inhibition of USP10 induces myeloma cell apoptosis by promoting cyclin D3 degradation

Authors - Yu-Jia Xu
humanWB

J Cell Mol Med

Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma

Authors - Xiao-Han Cui
humanIP,ChIP

Front Cell Dev Biol

RFWD3 Participates in the Occurrence and Development of Colorectal Cancer via E2F1 Transcriptional Regulation of BIRC5.

Authors - Fenghua Xu
humanWB

Ecotoxicol Environ Saf

The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.

Authors - Zemin Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Umut (Verified Customer) (08-30-2023)

It works very well, even though it is diluted a lot, i.e., 1:2000. The total protein concentration of my samples were around 2 ug/ml before adding 1:4 laemmli buffer and denature, and it is able to capture the target at low exposure times.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: U87MG, glioma-derived
E2F1 Antibody Western Blot validation (1:2000 dilution) in U87MG, glioma-derived (Cat no:66515-1-Ig)
FH

Juliane (Verified Customer) (05-11-2023)

The antibody worked fine, but only under using ECL sensitve reagent and high exposure when imaging.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
E2F1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:66515-1-Ig)