E2F1 Monoclonal antibody

• Featured Product
• KD/KO Validated

E2F1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IP, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

15

CloneNo.

5D7G8

Cat no : 66515-1-Ig

Synonyms

E2F 1, E2F transcription factor 1, E2F1, PBR3, PRB binding protein E2F 1, RBAP 1, RBAP1, RBBP 3, RBBP3, RBP3, Transcription factor E2F1

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Validation Data Gallery

• 

  WB analysis using 66515-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66515-1-Ig (E2F1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66515-1-Ig

  WB result of E2F1 antibody (66515-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E2F1 transfected HepG2 cells.

• 

  WB analysis using 66515-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66515-1-Ig (E2F1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66515-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66515-1-Ig (E2F1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 4 publications below

Product Information

66515-1-Ig targets E2F1 in WB, IP, IF, ChIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: E2F1 fusion protein Ag17363
• Full Name: E2F transcription factor 1
• Calculated molecular weight: 437 aa, 47 kDa
• Observed molecular weight: 55-60 kDa
• GenBank accession number: BC050369
• Gene symbol: E2F1
• Gene ID (NCBI): 1869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.

Publications

Species	Application	Title
human	chIP	Cell Death Dis; E2F1 mediated DDX11 transcriptional activation promotes hepatocellular carcinoma progression through PI3K/AKT/mTOR pathway.; Authors - Yan Yu
human	WB	FEBS Open Bio; Augmentation of the therapeutic efficacy of WEE1 kinase inhibitor AZD1775 by inhibiting the YAP-E2F1-DNA damage response pathway axis.; Authors - Yusuke Oku
human	WB,IF,ChIP	Cell Biol Int; FOXO1 inhibits prostate cancer cell proliferation via suppressing E2F1 activated NPRL2 expression; Authors - Yu Tang
human	WB	J Gene Med; Downregulation of lncRNA HCG11 promotes cell proliferation of oral squamous cell carcinoma through sponging miR-455-5p.; Authors - Jingjing Wu
human	ChIP	J Gene Med; Long non-coding RNA SNHG1 activates glycolysis to promote hepatocellular cancer progression through the miR-326/PKM2 axis.; Authors - Yue Wang
rat	WB	Front Physiol; Myricanol Inhibits Platelet Derived Growth Factor-BB-Induced Vascular Smooth Muscle Cells Proliferation and Migration in vitro and Intimal Hyperplasia in vivo by Targeting the Platelet-Derived Growth Factor Receptor-β and NF-κB Signaling.; Authors - Siyuan Fan