E2F1 Monoclonal antibody

• Featured Product
• KD/KO Validated

E2F1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

52

CloneNo.

5D7G8

Reviews (2)

Cat no : 66515-1-Ig

Synonyms

RBBP-3, RBAP-1, pRB-binding protein E2F-1, E2F-1, E2F 1

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Validation Data Gallery

• 

  WB analysis using 66515-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66515-1-Ig (E2F1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66515-1-Ig

  WB result of E2F1 antibody (66515-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E2F1 transfected HepG2 cells.

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Tested Applications

• Positive WB detected in: LNCaP cells, HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 43 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 3 publications below
• CoIP: See 3 publications below
• ChIP: See 10 publications below

Product Information

66515-1-Ig targets E2F1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: E2F1 fusion protein Ag17363
• Full Name: E2F transcription factor 1
• Calculated Molecular Weight: 437 aa, 47 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC050369
• Gene Symbol: E2F1
• Gene ID (NCBI): 1869
• RRID: AB_2881878
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.

Publications

Species	Application	Title
human	WB,IF	Cancer Res; LncRNA AGPG confers endocrine resistance in breast cancer by promoting E2F1 activity; Authors - Shiyi Yu
human	WB,IHC,IF,CoIP	Cell Death Dis; Identification of STAM-binding protein as a target for the treatment of gemcitabine resistance pancreatic cancer in a nutrient-poor microenvironment; Authors - Wenming Zhang ; KD Validated
human	WB	Cell Rep; FAK-mediated phosphorylation at Y464 regulates p85β nuclear translocation to promote tumorigenesis of ccRCC by repressing RB1 expression; Authors - Yanhua Zhang
human	WB	Acta Pharmacol Sin; Inhibition of USP10 induces myeloma cell apoptosis by promoting cyclin D3 degradation; Authors - Yu-Jia Xu
mouse	WB	J Ethnopharmacol; Classical prescription Floris Sophorae Powder treat colorectal cancer by regulating KRAS/MEK-ERK signaling pathway; Authors - Li-Ying Han
human	WB	J Cell Mol Med; Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma; Authors - Xiao-Han Cui

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Umut (Verified Customer) (08-30-2023)

It works very well, even though it is diluted a lot, i.e., 1:2000. The total protein concentration of my samples were around 2 ug/ml before adding 1:4 laemmli buffer and denature, and it is able to capture the target at low exposure times.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U87MG, glioma-derived

E2F1 Antibody Western Blot validation (1:2000 dilution) in U87MG, glioma-derived (Cat no:66515-1-Ig)

FH

Juliane (Verified Customer) (05-11-2023)

The antibody worked fine, but only under using ECL sensitve reagent and high exposure when imaging.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

E2F1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:66515-1-Ig)