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E2F1 Monoclonal antibody
E2F1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Human, mouse, rat and More (1)
WB, IP, IF, CoIP, ChIP, ELISA
Cat no : 66515-1-Ig
|Positive WB detected in||LNCaP cells, HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 23 publications below|
|IF||See 1 publications below|
|IP||See 1 publications below|
|CoIP||See 2 publications below|
|ChIP||See 8 publications below|
66515-1-Ig targets E2F1 in WB, IP, IF, CoIP, ChIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||E2F1 fusion protein Ag17363|
|Full Name||E2F transcription factor 1|
|Calculated molecular weight||437 aa, 47 kDa|
|Observed molecular weight||55-60 kDa|
|GenBank accession number||BC050369|
|Gene ID (NCBI)||1869|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.
|Product Specific Protocols|
|WB protocol for E2F1 antibody 66515-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
FAK-mediated phosphorylation at Y464 regulates p85β nuclear translocation to promote tumorigenesis of ccRCC by repressing RB1 expression
Acta Pharmacol Sin
Inhibition of USP10 induces myeloma cell apoptosis by promoting cyclin D3 degradation
J Cell Mol Med
Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma
Front Cell Dev Biol
RFWD3 Participates in the Occurrence and Development of Colorectal Cancer via E2F1 Transcriptional Regulation of BIRC5.
Ecotoxicol Environ Saf
The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.
Thymidine Kinase 1 Drives Skin Cutaneous Melanoma Malignant Progression and Metabolic Reprogramming.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Juliane (Verified Customer) (05-11-2023)
The antibody worked fine, but only under using ECL sensitve reagent and high exposure when imaging.