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E2F1 Monoclonal antibody

E2F1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, chIP, ELISA

Conjugate

Unconjugated

CloneNo.

5D7G8

Cat no : 66515-1-Ig

Synonyms

E2F 1, E2F transcription factor 1, E2F1, PBR3, PRB binding protein E2F 1, RBAP 1, RBAP1, RBBP 3, RBBP3, RBP3, Transcription factor E2F1



Tested Applications

Positive WB detected inLNCaP cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

66515-1-Ig targets E2F1 in WB, IHC, chIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen E2F1 fusion protein Ag17363
Full Name E2F transcription factor 1
Calculated molecular weight 437 aa, 47 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC050369
Gene symbol E2F1
Gene ID (NCBI) 1869
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.

Protocols

Product Specific Protocols
WB protocol for E2F1 antibody 66515-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Transl Med UniSa

siRNA Delivery for Control of Cyclin D1 and E2F1 Expression in Crohn's Disease.

Authors - Ilaria Russo
  • KD Validated
humanchIP

Cell Death Dis

E2F1 mediated DDX11 transcriptional activation promotes hepatocellular carcinoma progression through PI3K/AKT/mTOR pathway.

Authors - Yan Yu
humanWB

FEBS Open Bio

Augmentation of the therapeutic efficacy of WEE1 kinase inhibitor AZD1775 by inhibiting the YAP-E2F1-DNA damage response pathway axis.

Authors - Yusuke Oku