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E2F1 Antibody 2 Publications

Mouse Monoclonal| Catalog number: 66515-1-Ig |CloneNo.: 5D7G8

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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E2F1 Antibody WB HeLa cells 66515-1-Ig

Various cells were subjected to SDS PAGE followed by western blot with 66515-1-Ig (E2F1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

Species specificity:
Human, mouse, rat
Positive WB detected in:
HeLa,HEK-293, Jurkat, HSCT6, ROS1728 cells
Recommended dilution:
WB : 1:1000-1:3000

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
E2F1 fusion protein Ag17363
Full name:
E2F transcription factor 1
Calculated molecular weight:
437aa,47 kDa
Observed molecular weight:
55-60 kDa
GenBank accession number:
BC050369
Gene ID (NCBI):
1869
Gene symbol
E2F1
Synonyms
E2F 1, E2F transcription factor 1, E2F1, PBR3, PRB binding protein E2F 1, RBAP 1, RBAP1, RBBP 3, RBBP3, RBP3, Transcription factor E2F1

Background

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.


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