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E2F1 Monoclonal antibody
E2F1 Monoclonal Antibody for WB, ELISA
Cat no : 66515-1-Ig
|Positive WB detected in
|LNCaP cells, HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells
|Western Blot (WB)
|WB : 1:1000-1:6000
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66515-1-Ig targets E2F1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
|Human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Mouse / IgG2b
|E2F1 fusion protein Ag17363
|E2F transcription factor 1
|Calculated molecular weight
|437 aa, 47 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.
LncRNA AGPG confers endocrine resistance in breast cancer by promoting E2F1 activity
FAK-mediated phosphorylation at Y464 regulates p85β nuclear translocation to promote tumorigenesis of ccRCC by repressing RB1 expression
Acta Pharmacol Sin
Inhibition of USP10 induces myeloma cell apoptosis by promoting cyclin D3 degradation
J Cell Mol Med
Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma
Front Cell Dev Biol
RFWD3 Participates in the Occurrence and Development of Colorectal Cancer via E2F1 Transcriptional Regulation of BIRC5.
Ecotoxicol Environ Saf
The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Umut (Verified Customer) (08-30-2023)
It works very well, even though it is diluted a lot, i.e., 1:2000. The total protein concentration of my samples were around 2 ug/ml before adding 1:4 laemmli buffer and denature, and it is able to capture the target at low exposure times.
Juliane (Verified Customer) (05-11-2023)
The antibody worked fine, but only under using ECL sensitve reagent and high exposure when imaging.