E2F1 Monoclonal antibody, PBS Only

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  WB analysis using 66515-1-Ig (same clone as 66515-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66515-1-Ig (E2F1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66515-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66515-1-Ig (same clone as 66515-1-PBS)

  WB result of E2F1 antibody (66515-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E2F1 transfected HepG2 cells. This data was developed using the same antibody clone with 66515-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

E2F1 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 66515-1-PBS

Clone No.5D7G8

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

5D7G8, E2F 1, E2F-1, pRB-binding protein E2F-1, RBAP-1

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Product Information

66515-1-PBS targets E2F1 in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17363

  Product name: Recombinant human E2F1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 88-438 aa of BC050369

  Sequence: VKRRLDLETDHQYLAESSGPARGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSHTTVGVGGRLEGLTQDLRQLQESEQQLDHLMNICTTQLRLLSEDTDSQRLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAVDSSENFQISLKSKQGPIDVFLCPEETVGGISPGKTPSQEVTSEEENRATDSATIVSPPPSSPPSSLTTDPSQSLLSLEQEPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF

• Full Name: E2F transcription factor 1
• Calculated Molecular Weight: 437 aa, 47 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC050369
• Gene Symbol: E2F1
• Gene ID (NCBI): 1869
• RRID: AB_2881878
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q01094
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.