Tested Applications
| Positive WB detected in | mouse liver mitochondria tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17539-1-AP targets EARS2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11594 Product name: Recombinant human EARS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 184-534 aa of BC040013 Sequence: KPAIRFRLEQVVPAFQDLVYGWNRHEVASVEGDPVIMKSDGFPTYHLACVVDDHHMGISHVLRGSEWLVSTAKHLLLYQALGWQPPHFAHLPLLLNRDGSKLSKRQGDVFLEHFAADGFLPDSLLDIITNCGSGFAENQMGRTLPELITQFNLTQVTCHSALLDLEKLPEFNRLHLQRLVSNESQRRQLVGKLQVLVEEAFGCQLQNRDVLNPVYVERILLLRQGHICRLQDLVSPVYSYLWTRPAVGRAQLDAISEKVDVIAKRVLGLLERSGMSLTQDMLNGELKKLSEGLEGTKYSNVMKLLRMALSGQQVRQGHGLDCSLEPLIDPLNLHFLAGTELNIEYTKVNET Predict reactive species |
| Full Name | glutamyl-tRNA synthetase 2, mitochondrial (putative) |
| Calculated Molecular Weight | 534 aa, 60 kDa |
| Observed Molecular Weight | 59 kDa |
| GenBank Accession Number | BC040013 |
| Gene Symbol | EARS2 |
| Gene ID (NCBI) | 124454 |
| RRID | AB_2878414 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5JPH6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EARS2 encodes mitochondrial glutamyltRNA synthetase 2. Two isoforms were produced by alternative splicing with predicted MW of 58 and 60 kDa according to UniProt, and this antibody recognises both.
Publications
What published studies show
The EARS2 Polyclonal antibody (Cat# 17539-1-AP) has 1 citation to date. It appears in EMBO Molecular Medicine in a study on comprehensive functional testing in fibroblasts for diagnosing mitochondrial disease. The associated research field is mitochondrial disease and fibroblast-based diagnostics.
| Species | Application | Title |
|---|---|---|
EMBO Mol Med Comprehensive functional testing in fibroblasts has strong utility to diagnose mitochondrial disease. |
