EARS2 Polyclonal antibody

• 

  WB analysis of mouse liver mitochondria using 17539-1-AP

  mouse liver mitochondria tissue were subjected to SDS PAGE followed by western blot with 17539-1-AP (EARS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

EARS2 Polyclonal Antibody for WB, ELISA

Cat No. 17539-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EARS2, GluRS, Glutamate tRNA ligase, KIAA1970, MSE1

---

Tested Applications

• Positive WB detected in: mouse liver mitochondria tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17539-1-AP targets EARS2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11594

  Product name: Recombinant human EARS2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 184-534 aa of BC040013

  Sequence: KPAIRFRLEQVVPAFQDLVYGWNRHEVASVEGDPVIMKSDGFPTYHLACVVDDHHMGISHVLRGSEWLVSTAKHLLLYQALGWQPPHFAHLPLLLNRDGSKLSKRQGDVFLEHFAADGFLPDSLLDIITNCGSGFAENQMGRTLPELITQFNLTQVTCHSALLDLEKLPEFNRLHLQRLVSNESQRRQLVGKLQVLVEEAFGCQLQNRDVLNPVYVERILLLRQGHICRLQDLVSPVYSYLWTRPAVGRAQLDAISEKVDVIAKRVLGLLERSGMSLTQDMLNGELKKLSEGLEGTKYSNVMKLLRMALSGQQVRQGHGLDCSLEPLIDPLNLHFLAGTELNIEYTKVNET

• Full Name: glutamyl-tRNA synthetase 2, mitochondrial (putative)
• Calculated Molecular Weight: 534 aa, 60 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC040013
• Gene Symbol: EARS2
• Gene ID (NCBI): 124454
• RRID: AB_2878414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5JPH6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EARS2 encodes mitochondrial glutamyltRNA synthetase 2. Two isoforms were produced by alternative splicing with predicted MW of 58 and 60 kDa according to UniProt, and this antibody recognises both.