Tested Applications
| Positive WB detected in | COLO 320 cells, A431 cells, HeLa cells, HepG2 cells, K-562 cells, PC-3 cells |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16818-1-AP targets EED in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10358 Product name: Recombinant human EED protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-466 aa of BC068995 Sequence: MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSGRAILHSHQQCMRDPVSPNLRQHLSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR Predict reactive species |
| Full Name | embryonic ectoderm development |
| Calculated Molecular Weight | 466 aa, 53 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC068995 |
| Gene Symbol | EED |
| Gene ID (NCBI) | 8726 |
| RRID | AB_2262065 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75530 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for EED antibody 16818-1-AP | Download protocol |
| IF protocol for EED antibody 16818-1-AP | Download protocol |
| IHC protocol for EED antibody 16818-1-AP | Download protocol |
| IP protocol for EED antibody 16818-1-AP | Download protocol |
| WB protocol for EED antibody 16818-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EZH2 antibody (Cat# 16818-1-AP) has 22 citations. Publications appear in journals including Nature Communications, Blood, Molecular Cancer, Nature Structural & Molecular Biology, EMBO Journal, Cell Reports, Science Advances, Cell Death & Disease, Advanced Science, Analytical Chemistry, and others. Research fields span epigenetics and chromatin biology, PRC2/EZH2-mediated gene regulation, cancer (ovarian, AML, colon, lung, breast, oral), stem cell biology, neurotoxicity, and histone modification.
| Species | Application | Title |
|---|---|---|
Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer | ||
Blood Epigenetic Regulation of Non-canonical Menin Targets Modulates Menin Inhibitor Response in Acute Myeloid Leukemia | ||
Nat Commun Phosphorylation and stabilization of EZH2 by DCAF1/VprBP trigger aberrant gene silencing in colon cancer | ||
Adv Sci (Weinh) The Interplay Between HIF-1α and EZH2 in Lung Cancer and Dual-Targeted Drug Therapy | ||
Sci Adv Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells. | ||
Cell Death Dis Interplay of miR-137 and EZH2 contributes to the genome-wide redistribution of H3K27me3 underlying the Pb-induced memory impairment. |
Reviews
What customers say
The antibody received a 5-star rating in a Western Blot application. The reviewer used it at a 1:1000 dilution on HCT116 whole cell extract (20 µg/lane), with lanes 2–4 showing transient expression of EZH2. The results demonstrated clear and specific detection, confirming the antibody's reliability for WB. Overall, the review reflects a positive experience with strong performance and no reported issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nikhil (Verified Customer) (01-07-2022) | HCT116 whole cell extract 20microg/lane. Lane 2-4 transient expression of EZH2.
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