EED Polyclonal antibody

• Featured Product
• KD/KO Validated

EED Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (1)

Cat no : 16818-1-AP

Synonyms

WAIT-1, WAIT1, WAIT 1, Polycomb protein EED, Embryonic ectoderm development protein

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Validation Data Gallery

• 

  WB analysis of COLO 320 using 16818-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16818-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16818-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16818-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 16818-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16818-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 16818-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 16818-1-AP

  IP result of anti-EED (IP:16818-1-AP, 4ug; Detection:16818-1-AP 1:500) with PC-3 cells lysate 3200ug.

• 

  IHC staining of human tonsillitis using 16818-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16818-1-AP (EED Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 16818-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16818-1-AP (EED Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16818-1-AP

  Immunofluorescent analysis of (10% Formalin) fixed HepG2 cells using 16818-1-AP (EED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 16818-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16818-1-AP (EED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 16818-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human EED (16818-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: COLO 320 cells, A431 cells, HeLa cells, HepG2 cells, K-562 cells, PC-3 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 19 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

16818-1-AP targets EED in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EED fusion protein Ag10358
• Full Name: embryonic ectoderm development
• Calculated Molecular Weight: 466 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC068995
• Gene Symbol: EED
• Gene ID (NCBI): 8726
• RRID: AB_2262065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.

Publications

Species	Application	Title
human	WB	Mol Cancer; Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer; Authors - Rashmi Bharti
human	WB	Blood; Epigenetic Regulation of Non-canonical Menin Targets Modulates Menin Inhibitor Response in Acute Myeloid Leukemia; Authors - Xinyue Zhou
human	WB	EMBO J; A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.; Authors - Xu Wang
human	WB	Eur J Med Chem; Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.; Authors - Cheng Wang
		J Cell Sci; EZH1/SUZ12 complex positively regulates the transcription of NF-κB target genes via interaction with UXT.; Authors - Shuai-Kun Su
human	WB	Sci Rep; Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1.; Authors - Masamitsu N Asaka

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nikhil (Verified Customer) (01-07-2022)

HCT116 whole cell extract 20microg/lane. Lane 2-4 transient expression of EZH2.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT116

EED Antibody Western Blot validation (1:1000 dilution) in HCT116 (Cat no:16818-1-AP)

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