|Positive WB detected in||COLO 320 cells, A431 cells, HeLa cells, HepG2 cells, K-562 cells, PC-3 cells|
|Positive IP detected in||PC-3 cells|
|Positive IHC detected in||human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16818-1-AP targets EED in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EED fusion protein Ag10358|
|Full Name||embryonic ectoderm development|
|Calculated molecular weight||466 aa, 53 kDa|
|Observed molecular weight||53 kDa|
|GenBank accession number||BC068995|
|Gene ID (NCBI)||8726|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.
|Product Specific Protocols|
|WB protocol for EED antibody 16818-1-AP||Download protocol|
|IHC protocol for EED antibody 16818-1-AP||Download protocol|
|IF protocol for EED antibody 16818-1-AP||Download protocol|
|IP protocol for EED antibody 16818-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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