EED Polyclonal antibody

EED Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16818-1-AP

Synonyms

EED, embryonic ectoderm development, HEED, Polycomb protein EED, WAIT 1, WAIT1



Tested Applications

Positive WB detected inCOLO 320 cells, A431 cells, HeLa cells, HepG2 cells, K-562 cells, PC-3 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

16818-1-AP targets EED in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EED fusion protein Ag10358
Full Name embryonic ectoderm development
Calculated molecular weight 466 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC068995
Gene symbol EED
Gene ID (NCBI) 8726
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.

Protocols

Product Specific Protocols
WB protocol for EED antibody 16818-1-APDownload protocol
IHC protocol for EED antibody 16818-1-APDownload protocol
IF protocol for EED antibody 16818-1-APDownload protocol
IP protocol for EED antibody 16818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Int J Biol Sci

Identification and Functional Characterization of a New Splicing Variant of EZH2 in the Central Nervous System.

Authors - Danyang Li
humanWB

Sci Adv

Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells.

Authors - Xiangxiang Wei
ratWB

Cell Death Dis

Interplay of miR-137 and EZH2 contributes to the genome-wide redistribution of H3K27me3 underlying the Pb-induced memory impairment.

Authors - Xiaozhen Gu
humanWB

Oncotarget

Multiple regulatory aspects of histone methyltransferase EZH2 in Pb-induced neurotoxicity.

Authors - Wei-Zhen Xue
humanWB

Sci Rep

Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1.

Authors - Masamitsu N Asaka

J Cell Sci

EZH1/SUZ12 complex positively regulates the transcription of NF-κB target genes via interaction with UXT.

Authors - Shuai-Kun Su