PHD2/EGLN1 Monoclonal antibody

PHD2/EGLN1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat , Pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A2F1

Cat no : 66589-1-Ig

Synonyms

C1orf12, ECYT3, Egl nine homolog 1, EGLN1, HIF PH2, HIF prolyl hydroxylase 2, HIFPH2, HPH 2, HPH2, PHD2, PNAS 137, SM 20, SM20, ZMYND6



Tested Applications

Positive WB detected inmouse brain tissue, SH-SY5Y cells, pig brain tissue, HEK-293 cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66589-1-Ig targets PHD2/EGLN1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse , Rat , Pig samples.

Tested Reactivity Human, Mouse , Rat , Pig
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name egl nine homolog 1 (C. elegans)
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa, 44 kDa, 36 kDa
GenBank accession numberNM_022051
Gene symbol EGLN1
Gene ID (NCBI) 54583
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.

Protocols

Product Specific Protocols
WB protocol for PHD2/EGLN1 antibody 66589-1-IgDownload protocol
IHC protocol for PHD2/EGLN1 antibody 66589-1-IgDownload protocol
IF protocol for PHD2/EGLN1 antibody 66589-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

Hypoxia-Inducible Factor 2-Alpha Mediated Gene Sets Differentiate Pulmonary Arterial Hypertension.

Authors - Jinsheng Zhu