PHD2/EGLN1 Monoclonal antibody

• Featured Product
• KD/KO Validated

PHD2/EGLN1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1A2F1

Cat no : 66589-1-Ig

Synonyms

EGLN1, PHD2, EGLN 1, ECYT3, EC:1.14.11.29

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Validation Data Gallery

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  WB analysis of mouse brain using 66589-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66589-1-Ig (EGLN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66589-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66589-1-Ig (EGLN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 66589-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66589-1-Ig (EGLN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66589-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66589-1-Ig (EGLN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 66589-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66589-1-Ig (EGLN1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66589-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66589-1-Ig (EGLN1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66589-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66589-1-Ig (EGLN1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66589-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66589-1-Ig (EGLN1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 66589-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PHD2/EGLN1 antibody (66589-1-Ig, Clone: 1A2F1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, SH-SY5Y cells, pig brain tissue, HEK-293 cells
• Positive IHC detected in: human testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

66589-1-Ig targets PHD2/EGLN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: egl nine homolog 1 (C. elegans)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa, 44 kDa, 36 kDa
• GenBank Accession Number: NM_022051
• Gene Symbol: PHD2/EGLN1
• Gene ID (NCBI): 54583
• RRID: AB_2881949
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.

Publications

Species	Application	Title
mouse	WB	Front Cell Dev Biol; Hypoxia-Inducible Factor 2-Alpha Mediated Gene Sets Differentiate Pulmonary Arterial Hypertension.; Authors - Jinsheng Zhu
mouse	WB	J Transl Med; Inhibition of OGFOD1 by FG4592 confers neuroprotection by activating unfolded protein response and autophagy after ischemic stroke; Authors - Jian Xie ; KD Validated