PHD2/EGLN1 Monoclonal antibody

PHD2/EGLN1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human, Mouse , Rat , Pig and More (1)







Cat no : 66589-1-Ig


C1orf12, ECYT3, Egl nine homolog 1, EGLN1, HIF PH2, HIF prolyl hydroxylase 2, HIFPH2, HPH 2, HPH2, PHD2, PNAS 137, SM 20, SM20, ZMYND6

Tested Applications

Positive WB detected inmouse brain tissue, SH-SY5Y cells, pig brain tissue, HEK-293 cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66589-1-Ig targets PHD2/EGLN1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse , Rat , Pig samples.

Tested Reactivity Human, Mouse , Rat , Pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name egl nine homolog 1 (C. elegans)
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa, 44 kDa, 36 kDa
GenBank Accession NumberNM_022051
Gene Symbol EGLN1
Gene ID (NCBI) 54583
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.


Product Specific Protocols
WB protocol for PHD2/EGLN1 antibody 66589-1-IgDownload protocol
IHC protocol for PHD2/EGLN1 antibody 66589-1-IgDownload protocol
IF protocol for PHD2/EGLN1 antibody 66589-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Cell Dev Biol

Hypoxia-Inducible Factor 2-Alpha Mediated Gene Sets Differentiate Pulmonary Arterial Hypertension.

Authors - Jinsheng Zhu

J Transl Med

Inhibition of OGFOD1 by FG4592 confers neuroprotection by activating unfolded protein response and autophagy after ischemic stroke

Authors - Jian Xie
  • KD Validated