CoraLite® Plus 488-conjugated PHD2/EGLN1 Monoclonal antibody

PHD2/EGLN1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat , Pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A2F1

Cat no : CL488-66589

Synonyms

C1orf12, DKFZp761F179, ECYT3, Egl nine homolog 1, EGLN1, HIF PH2, HIF prolyl hydroxylase 2, HIFPH2, HPH 2, HPH2, PHD2, PHD2/EGLN1, PNAS 137, SM 20, SM20, ZMYND6

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Validation Data Gallery

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  IF Staining of HEK-293 using CL488-66589

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®®488 PHD2/EGLN1 antibody (CL488-66589, Clone: 1A2F1 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66589 targets PHD2/EGLN1 in IF applications and shows reactivity with Human, Mouse , Rat , Pig samples.

• Tested Reactivity: Human, Mouse , Rat , Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: egl nine homolog 1 (C. elegans)
• Calculated Molecular Weight: 46 kDa
• GenBank Accession Number: NM_022051
• Gene Symbol: EGLN1
• Gene ID (NCBI): 54583
• RRID: AB_2919349
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.