PHD2/EGLN1 Monoclonal antibody, PBS Only

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  WB analysis of mouse brain using 66589-1-Ig (same clone as 66589-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66589-1-Ig (EGLN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66589-1-Ig (same clone as 66589-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66589-1-Ig (EGLN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 66589-1-Ig (same clone as 66589-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66589-1-Ig (EGLN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66589-1-Ig (same clone as 66589-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66589-1-Ig (EGLN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66589-1-Ig (same clone as 66589-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66589-1-Ig (EGLN1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66589-1-Ig (same clone as 66589-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66589-1-Ig (EGLN1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66589-1-Ig (same clone as 66589-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66589-1-Ig (EGLN1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66589-1-Ig (same clone as 66589-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66589-1-Ig (EGLN1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 66589-1-Ig (same clone as 66589-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PHD2/EGLN1 antibody (66589-1-Ig, Clone: 1A2F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PHD2/EGLN1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66589-1-PBS

Clone No.1A2F1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EGLN1, PHD2, EGLN 1, ECYT3, EC:1.14.11.29

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Product Information

66589-1-PBS targets PHD2/EGLN1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: egl nine homolog 1 (C. elegans)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa, 44 kDa, 36 kDa
• GenBank Accession Number: NM_022051
• Gene Symbol: PHD2/EGLN1
• Gene ID (NCBI): 54583
• RRID: AB_2881949
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9GZT9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.