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  • KD/KO Validated

PHD3 Polyclonal antibody

PHD3 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 18325-1-AP

Synonyms

Egl nine homolog 3, EGLN3, HIF PH3, HIF prolyl hydroxylase 3, HIFPH3, HPH 1, HPH 3, PHD3



Tested Applications

Positive WB detected inHT-1080 cells, A375 cells, HEK-293 cells, Jurkat cells
Positive IP detected inA375 cells
Positive IHC detected inhuman brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18325-1-AP is PHD3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHD3 fusion protein Ag13197
Full Name egl nine homolog 3 (C. elegans)
Calculated molecular weight 27 kDa
Observed molecular weight 28-32 kDa
GenBank accession numberBC010992
Gene symbol EGLN3
Gene ID (NCBI) 112399
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.

Protocols

Product Specific Protocols
WB protocol for PHD3 antibody 18325-1-APDownload protocol
IHC protocol for PHD3 antibody 18325-1-APDownload protocol
IP protocol for PHD3 antibody 18325-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Thorac Dis

Prolyl hydroxylase 3 involvement in lung cancer progression under hypoxic conditions: association with hypoxia-inducible factor-1α and pyruvate kinase M2.

Authors - Xiao Chu
humanIHC

Med Oncol

Overexpression of the HIF hydroxylase PHD3 is a favorable prognosticator for gastric cancer.

Authors - Su Changlei C
humanWB

Mol Ther

WDR5 facilitates EMT and metastasis of CCA by increasing HIF-1α accumulation in Myc-dependent and independent pathways.

Authors - Tianli Chen
  • KD Validated
humanIHC

Cancer Res

Prolyl Hydroxylase 3 Attenuates MCL-1-Mediated ATP Production to Suppress the Metastatic Potential of Colorectal Cancer Cells.

Authors - Praveenkumar Radhakrishnan