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  • KD/KO Validated

EIF2A/CDA02 Polyclonal antibody

EIF2A/CDA02 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11233-1-AP

Synonyms

CDA02, EIF 2A, EIF2A, MST089, MSTP004, MSTP089



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, Raji cells, PC-3 cells, mouse pancreas tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman prostate cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Ethacrynic acid treated HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11233-1-AP targets EIF2A/CDA02 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF2A/CDA02 fusion protein Ag1753
Full Name eukaryotic translation initiation factor 2A, 65kDa
Calculated molecular weight 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC011885
Gene symbol EIF2A
Gene ID (NCBI) 83939
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EIF2A, recognizes specifically the 65kd EIF2A protein.

Protocols

Product Specific Protocols
WB protocol for EIF2A/CDA02 antibody 11233-1-APDownload protocol
IHC protocol for EIF2A/CDA02 antibody 11233-1-APDownload protocol
IF protocol for EIF2A/CDA02 antibody 11233-1-APDownload protocol
IP protocol for EIF2A/CDA02 antibody 11233-1-APDownload protocol
FC protocol for EIF2A/CDA02 antibody 11233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

CNS Neurosci Ther

Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway.

Authors - Ming-Feng Zhou
ratWB

Br J Pharmacol

Hydrogen Sulfide Regulates Muscle RING Finger-1 Protein S-Sulfhydration at Cys44 to Prevent Cardiac Structural Damage in Diabetic Cardiomyopathy.

Authors - Xiaojiao Sun
humanWB

Front Immunol

Evaluation of the Antiviral Activity of Sephin1 Treatment and Its Consequences on eIF2α Phosphorylation in Response to Viral Infections.

Authors - Maxime Fusade-Boyer
humanWB

J Cell Mol Med

PMID: 31211507

Authors - Lin Chen
  • KD Validated
mouseWB

Biosci Rep

PMID: 30181379

Authors - Yonghua Chen
humanWB

World J Gastrointest Oncol

KMT2D deficiency enhances the anti-cancer activity of L48H37 in pancreatic ductal adenocarcinoma.

Authors - Si-Si Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Daniel (Verified Customer) (04-01-2019)

The provided antibody exceeded all expectations. It worked very well on the first try. Mouse liver was homogenized in Final sample buffer, and then proteins were separated by 10% SDS-PAGE followed by western blot with 11233-1-AP(EIF2A antibody) at a dilution of 1:1000 incubated at 4 degrees Celsius over night in 5% BSA TBST.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Liver
EIF2A/CDA02 Antibody Western Blot, validation (1:1000 dilution) in Mouse Liver (Cat no:11233-1-AP)
FH

Sahya (Verified Customer) (08-13-2018)

Showed a very strong signal, will use at a higher dilution next time.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Skeletal Muscle Satellite Cells