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EIF2A/CDA02 Polyclonal antibody
EIF2A/CDA02 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 11233-1-AP
Validation Data Gallery
|Positive WB detected in||MCF-7 cells, HeLa cells, Raji cells, PC-3 cells, mouse pancreas tissue|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human prostate cancer tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, Ethacrynic acid treated HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11233-1-AP targets EIF2A/CDA02 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EIF2A/CDA02 fusion protein Ag1753|
|Full Name||eukaryotic translation initiation factor 2A, 65kDa|
|Calculated molecular weight||65 kDa|
|Observed molecular weight||65 kDa|
|GenBank accession number||BC011885|
|Gene ID (NCBI)||83939|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EIF2A, recognizes specifically the 65kd EIF2A protein.
|Product Specific Protocols|
|WB protocol for EIF2A/CDA02 antibody 11233-1-AP||Download protocol|
|IHC protocol for EIF2A/CDA02 antibody 11233-1-AP||Download protocol|
|IF protocol for EIF2A/CDA02 antibody 11233-1-AP||Download protocol|
|IP protocol for EIF2A/CDA02 antibody 11233-1-AP||Download protocol|
|FC protocol for EIF2A/CDA02 antibody 11233-1-AP||Download protocol|
|Click here to view our Standard Protocols|
CNS Neurosci Ther
Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway.
Br J Pharmacol
Hydrogen Sulfide Regulates Muscle RING Finger-1 Protein S-Sulfhydration at Cys44 to Prevent Cardiac Structural Damage in Diabetic Cardiomyopathy.
Evaluation of the Antiviral Activity of Sephin1 Treatment and Its Consequences on eIF2α Phosphorylation in Response to Viral Infections.
J Cell Mol Med
World J Gastrointest Oncol
KMT2D deficiency enhances the anti-cancer activity of L48H37 in pancreatic ductal adenocarcinoma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Daniel (Verified Customer) (04-01-2019)
The provided antibody exceeded all expectations. It worked very well on the first try. Mouse liver was homogenized in Final sample buffer, and then proteins were separated by 10% SDS-PAGE followed by western blot with 11233-1-AP(EIF2A antibody) at a dilution of 1:1000 incubated at 4 degrees Celsius over night in 5% BSA TBST.
Sahya (Verified Customer) (08-13-2018)
Showed a very strong signal, will use at a higher dilution next time.