• Featured Product
  • KD/KO Validated

EIF2A/CDA02 Polyclonal antibody

EIF2A/CDA02 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11233-1-AP

Synonyms

CDA02, EIF 2A, EIF2A, EIF2A/CDA02, Eukaryotic translation initiation factor 2A, MST089, MSTP004, MSTP089



Tested Applications

Positive WB detected inMCF-7 cells, HEK-293T cells, HeLa cells, PC-12 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman prostate cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Ethacrynic acid treated HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11233-1-AP targets EIF2A/CDA02 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF2A/CDA02 fusion protein Ag1753
Full Name eukaryotic translation initiation factor 2A, 65kDa
Calculated molecular weight 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC011885
Gene symbol EIF2A
Gene ID (NCBI) 83939
RRIDAB_2246321
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EIF2A, recognizes specifically the 65kd EIF2A protein. EIF2A (Gene ID: 83939) and EIF2S1 (Gene ID: 1965) share the EIF2A symbol/alias in common. EIF2S1 is the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40S ribosomal subunit, the EIF2A protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP.

Protocols

Product Specific Protocols
WB protocol for EIF2A/CDA02 antibody 11233-1-APDownload protocol
IHC protocol for EIF2A/CDA02 antibody 11233-1-APDownload protocol
IF protocol for EIF2A/CDA02 antibody 11233-1-APDownload protocol
IP protocol for EIF2A/CDA02 antibody 11233-1-APDownload protocol
FC protocol for EIF2A/CDA02 antibody 11233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell

Circadian clocks are modulated by compartmentalized oscillating translation

Authors - Yanrong Zhuang
mouseWB

Nature

Translation from unconventional 5' start sites drives tumour initiation.

Authors - Ataman Sendoel
  • KO Validated
WB

Science

Translation from the 5' untranslated region shapes the integrated stress response.

Authors - Shelley R Starck
  • KD Validated
humanWB

Science

Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I.

Authors - Shelley R Starck
  • KD Validated
humanWB

Sci Adv

CReP mediates selective translation initiation at the endoplasmic reticulum.

Authors - Jonathan P Kastan
mouseWB

J Clin Invest

Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.

Authors - Zhixiong Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Gianluca (Verified Customer) (11-25-2021)

Good scientific feedback

  • Applications: Western Blot
FH

Daniel (Verified Customer) (04-01-2019)

The provided antibody exceeded all expectations. It worked very well on the first try.Mouse liver was homogenized in Final sample buffer, and then proteins were separated by 10% SDS-PAGE followed by western blot with 11233-1-AP(EIF2A antibody) at a dilution of 1:1000 incubated at 4 degrees Celsius over night in 5% BSA TBST.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Liver
EIF2A/CDA02 Antibody Western Blot, validation (1:1000 dilution) in Mouse Liver (Cat no:11233-1-AP)
FH

Sahya (Verified Customer) (08-13-2018)

Showed a very strong signal, will use at a higher dilution next time.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Skeletal Muscle Satellite Cells