EIF3D Recombinant antibody

EIF3D Recombinant Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3O8

Cat no : 82512-1-RR

Synonyms

eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7

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Validation Data Gallery

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  WB analysis using 82512-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82512-1-RR (EIF3D antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 82512-1-RR

  rat liver tissue were subjected to SDS PAGE followed by western blot with 82512-1-RR (EIF3D antibody) at dilution of 1:30300 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 82512-1-RR

  IP result of anti-EIF3D (IP:82512-1-RR, 4ug; Detection:82512-1-RR 1:5000) with A549 cells lysate 920 ug.

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  IHC staining of human breast cancer using 82512-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82512-1-RR (EIF3D antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human breast cancer using 82512-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82512-1-RR (EIF3D antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

• 

  IF Staining of U2OS using 82512-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using EIF3D antibody (82512-1-RR, Clone: 3O8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, rat liver tissue, HepG2 cells, A549 cells, A431 cells, Jurkat cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82512-1-RR targets EIF3D in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EIF3D fusion protein Ag0268
• Full Name: eukaryotic translation initiation factor 3, subunit D
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC000328
• Gene Symbol: EIF3D
• Gene ID (NCBI): 8664
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.