EIF3D Recombinant antibody

EIF3D Recombinant Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


Human, rat







Cat no : 82512-1-RR


eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7

Tested Applications

Positive WB detected inHeLa cells, rat liver tissue, HepG2 cells, A549 cells, A431 cells, Jurkat cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82512-1-RR targets EIF3D in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EIF3D fusion protein Ag0268
Full Name eukaryotic translation initiation factor 3, subunit D
Calculated Molecular Weight 66 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC000328
Gene Symbol EIF3D
Gene ID (NCBI) 8664
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.


Product Specific Protocols
WB protocol for EIF3D antibody 82512-1-RRDownload protocol
IHC protocol for EIF3D antibody 82512-1-RRDownload protocol
IF protocol for EIF3D antibody 82512-1-RRDownload protocol
IP protocol for EIF3D antibody 82512-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols