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EIF3M Antibody

EIF3M Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11423-1-AP

Synonyms

B5, EIF3M, Fetal lung protein B5, GA17, hfl B5, HFLB5, PCID1, PNAS 125



Tested Applications

Positive WB detected inHeLa cells, mouse uterus tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11423-1-AP targets EIF3M in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF3M fusion protein Ag1994
Full Name eukaryotic translation initiation factor 3, subunit M
Calculated molecular weight 374 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC019103
Gene symbol EIF3M
Gene ID (NCBI) 10480
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.

Protocols

Product Specific Protocols
WB protocol for EIF3M antibody 11423-1-APDownload protocol
IHC protocol for EIF3M antibody 11423-1-APDownload protocol
IF protocol for EIF3M antibody 11423-1-APDownload protocol
IP protocol for EIF3M antibody 11423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Oncogene

eIF3m expression influences the regulation of tumorigenesis-related genes in human colon cancer.

Authors - Goh S-H SH
  • KD Validated
humanWB

Nature

Genome-wide silencing in Drosophila captures conserved apoptotic effectors.

Authors - Chew Su Kit SK