• Featured Product
  • KD/KO Validated

EIF3M Polyclonal antibody

EIF3M Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 11423-1-AP


B5, EIF3M, Fetal lung protein B5, GA17, hfl B5, HFLB5, PCID1, PNAS 125

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11423-1-AP targets EIF3M in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF3M fusion protein Ag1994
Full Name eukaryotic translation initiation factor 3, subunit M
Calculated Molecular Weight 374 aa, 43 kDa
Observed Molecular Weight 35-43 kDa
GenBank Accession NumberBC019103
Gene Symbol EIF3M
Gene ID (NCBI) 10480
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.


Product Specific Protocols
WB protocol for EIF3M antibody 11423-1-APDownload protocol
IHC protocol for EIF3M antibody 11423-1-APDownload protocol
IF protocol for EIF3M antibody 11423-1-APDownload protocol
IP protocol for EIF3M antibody 11423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Genome-wide silencing in Drosophila captures conserved apoptotic effectors.

Authors - Chew Su Kit SK

J Cell Biol

Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine.

Authors - Chen Chen


eIF3m expression influences the regulation of tumorigenesis-related genes in human colon cancer.

Authors - Goh S-H SH
  • KD Validated

Am J Cancer Res

EIF3m promotes the malignant phenotype of lung adenocarcinoma by the up-regulation of oncogene CAPRIN1.

Authors - Xinwei Liu
  • KD Validated


Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges

Authors - Tamami Miyagi