|Positive WB detected in||HeLa cells, HepG2 cells, MCF-7 cells, mouse brain tissue, rat brain tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11423-1-AP targets EIF3M in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EIF3M fusion protein Ag1994|
|Full Name||eukaryotic translation initiation factor 3, subunit M|
|Calculated molecular weight||374 aa, 43 kDa|
|Observed molecular weight||35-43 kDa|
|GenBank accession number||BC019103|
|Gene ID (NCBI)||10480|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.
|Product Specific Protocols|
|WB protocol for EIF3M antibody 11423-1-AP||Download protocol|
|IHC protocol for EIF3M antibody 11423-1-AP||Download protocol|
|IF protocol for EIF3M antibody 11423-1-AP||Download protocol|
|IP protocol for EIF3M antibody 11423-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Genome-wide silencing in Drosophila captures conserved apoptotic effectors.
J Cell Biol
Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine.
eIF3m expression influences the regulation of tumorigenesis-related genes in human colon cancer.
Am J Cancer Res
EIF3m promotes the malignant phenotype of lung adenocarcinoma by the up-regulation of oncogene CAPRIN1.
Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges