EIF4G1 Polyclonal antibody

EIF4G1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15704-1-AP

Synonyms

eIF 4 gamma 1, eIF 4G 1, eIF 4G1, EIF4F, EIF4G, EIF4G1, EIF4GI, p220



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells, Ethacrynic acid treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15704-1-AP targets EIF4G1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF4G1 fusion protein Ag8342
Full Name eukaryotic translation initiation factor 4 gamma, 1
Calculated molecular weight 1600 aa, 176 kDa
Observed molecular weight 250 kDa
GenBank accession numberBC007788
Gene symbol EIF4G1
Gene ID (NCBI) 1981
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )

Protocols

Product Specific Protocols
WB protocol for EIF4G1 antibody 15704-1-APDownload protocol
IHC protocol for EIF4G1 antibody 15704-1-APDownload protocol
IF protocol for EIF4G1 antibody 15704-1-APDownload protocol
IP protocol for EIF4G1 antibody 15704-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Mol Genet Genomic Med

EIF4G1 is a novel candidate gene associated with severe asthenozoospermia.

Authors - Yanwei Sha
humanWB

J Virol Methods

Construction of a subgenomic CV-B3 replicon expressing emerald green fluorescent protein to assess viral replication of a cardiotropic enterovirus strain in cultured human cells.

Authors - Michel Wehbe
humanWB

Virol J

Protease 2A induces stress granule formation during coxsackievirus B3 and enterovirus 71 infections.

Authors - Shuo Wu
humanWB,IHC

Neurochem Res

ALS-Linked Mutant SOD1 Associates with TIA-1 and Alters Stress Granule Dynamics.

Authors - Do-Yeon Lee
mouseWB,IF

Diabetes

Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function.

Authors - Seokwon Jo
  • KO Validated
humanWB

PLoS Genet

SAMHD1 Inhibits LINE-1 Retrotransposition by Promoting Stress Granule Formation.

Authors - Siqi Hu