Tested Applications
| Positive WB detected in | BxPC-3 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, Ethacrynic acid treated HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15704-1-AP targets EIF4G1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8342 Product name: Recombinant human EIF4G1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 296-645 aa of BC007788 Sequence: IEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN Predict reactive species |
| Full Name | eukaryotic translation initiation factor 4 gamma, 1 |
| Calculated Molecular Weight | 1600 aa, 176 kDa |
| Observed Molecular Weight | 230-250 kDa |
| GenBank Accession Number | BC007788 |
| Gene Symbol | EIF4G1 |
| Gene ID (NCBI) | 1981 |
| RRID | AB_2261979 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q04637 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF4G1 antibody 15704-1-AP | Download protocol |
| IHC protocol for EIF4G1 antibody 15704-1-AP | Download protocol |
| IP protocol for EIF4G1 antibody 15704-1-AP | Download protocol |
| WB protocol for EIF4G1 antibody 15704-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EIF4G1 Polyclonal antibody (Cat# 15704-1-AP) has 47 citations. These appear in high-impact journals including Nature, Nature Communications, Molecular Cell, PNAS, Developmental Cell, EMBO Journal, Cell Reports, Advanced Science, and Oncogene, among others. Research fields span translation regulation, stress granule dynamics, cancer biology (hepatocellular, breast, gastric, bladder), viral infection (Coxsackievirus, reovirus), glucose homeostasis, neurodegeneration (ALS), and immune regulation. Applications include WB, IF, IP, CoIP, IHC, and ELISA.
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Extracellular vesicles rich in HAX1 promote angiogenesis by modulating ITGB6 translation. | ||
Nat Commun The protein circPETH-147aa regulates metabolic reprogramming in hepatocellular carcinoma cells to remodel immunosuppressive microenvironment | ||
Mol Cell The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation. | ||
Mol Cell The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance |
