ELP3 Polyclonal antibody

ELP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17016-1-AP

Synonyms

Elongator complex protein 3, ELP3, hELP3, KAT9

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Validation Data Gallery

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  WB analysis using 17016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17016-1-AP (ELP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 17016-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17016-1-AP (ELP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17016-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17016-1-AP (ELP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 17016-1-AP is ELP3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ELP3 fusion protein Ag9993
• Full Name: elongation protein 3 homolog (S. cerevisiae)
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 60-62 kDa
• GenBank Accession Number: BC001240
• Gene Symbol: ELP3
• Gene ID (NCBI): 55140
• RRID: AB_2918048
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	bioRxiv; TDP-43 nuclear loss in FTD/ALS causes widespread alternative polyadenylation changes; Authors - Yi Zeng
human	IHC	iScience; RNA modification-related genes illuminate prognostic signature and mechanism in esophageal squamous cell carcinoma; Authors - Hui Han
human	WB	J Clin Invest; CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes; Authors - Nunziata Maio